Phosphorylated IkappaBα is highly enriched in the axon initial segment

Schultz, C., König, H. G., Del Turco, D., Dehn, D., Ghebremedhin, E., Prehn, J. H. M., Kögel, D. and Deller, T. (2004). Phosphorylated IkappaBα is highly enriched in the axon initial segment. In: The 34th Annual Meeting of Neuroscience, San Diego, California, (). 23-27 October 2004.

Author Schultz, C.
König, H. G.
Del Turco, D.
Dehn, D.
Ghebremedhin, E.
Prehn, J. H. M.
Kögel, D.
Deller, T.
Title of paper Phosphorylated IkappaBα is highly enriched in the axon initial segment
Conference name The 34th Annual Meeting of Neuroscience
Conference location San Diego, California
Conference dates 23-27 October 2004
Publisher Society for Neuroscience
Publication Year 2004
Sub-type Poster
Language eng
Abstract/Summary The transcription factor NF-KappaB (NF-KB) is present in the cytosol in an inactive state, complexed with the inhibitory protein IKappaBα. Constitutive activation of NF-KB occurs in neurons of the central nervous system via phosphorylation of IKappaBα at Ser32 and Ser36 and subsequent degradation of phospho-IKappaBα. We studied this constitutive neuronal activation of NF-KB in brain sections of several mammals (mice, rats, rhesus monkeys, humans) using immunostaining with polyclonal and monoclonal antibodies directed against IKappaBα phosphorylated at Ser32 and Ser36. Strikingly, a highly selective enrichment of phospho-IKappaBα was found in the axon initial segment of all species examined. Specificity of the immunostaining was demonstrated by preincubating the anti-phospho-IKappaBα antibodies with the corresponding phospho-IKappaBα blocking peptides which completely prevented immunolabeling. The selective axonal localization of phospho-IKappaBα was confirmed by double-immunostaining with antibodies directed against the axonal protein tau and the dendritic microtubule-associated protein 2 (MAP2). Distal segments of axons, dendrites and cell bodies were either negative for phospho-IKappaBα or only faintly labeled. Likewise, glial cells were phospho-IKappaBα-negative. A similiar selective localization of phospho-IKappaBα in the axon initial segment was found in organotypic slice cultures and dissociated primary neuron cultures prepared from rat hippocampi. Taken together, these findings implicate the axon initial segment as a cell compartment which is crucial for neuronal activation of NF-KB.
Subjects 1103 Clinical Sciences
Keyword Axon
Transcription factor
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Presentation Number: 38.9 Session: 38. Cytokines and Other Factors I

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Created: Wed, 03 Mar 2010, 10:48:01 EST by Laura McTaggart on behalf of Faculty of Science