Presence of murine organic anion transporter 1(mOAT1) in kidneys and brain and interaction with tryptophan metabolites

Hagos, Y., Lorenz, H., Ghebremedhin, E., Schultz, C., Ljubojevic, M., Sabolic, I., Burckhardt, G. and Bahn, A. (2004). Presence of murine organic anion transporter 1(mOAT1) in kidneys and brain and interaction with tryptophan metabolites. In: Pflügers Archiv European Journal of Physiology. 83rd Annual Meeting DPG. 83rd Annual Meeting of the German Physiological Society (DPG), Leipzig, Germany, (S72-S72). 14-17 March 2004. doi:10.1007/s00424-004-1271-8


Author Hagos, Y.
Lorenz, H.
Ghebremedhin, E.
Schultz, C.
Ljubojevic, M.
Sabolic, I.
Burckhardt, G.
Bahn, A.
Title of paper Presence of murine organic anion transporter 1(mOAT1) in kidneys and brain and interaction with tryptophan metabolites
Conference name 83rd Annual Meeting of the German Physiological Society (DPG)
Conference location Leipzig, Germany
Conference dates 14-17 March 2004
Proceedings title Pflügers Archiv European Journal of Physiology. 83rd Annual Meeting DPG   Check publisher's open access policy
Place of Publication Berlin / Heidelberg, Germany
Publisher Springer
Publication Year 2004
Sub-type Poster
DOI 10.1007/s00424-004-1271-8
ISSN 0031-6768
1432-2013
Volume 447
Issue Supplement 1
Start page S72
End page S72
Total pages 1
Language eng
Abstract/Summary Organic anion transporters (OATs) facilitate the renal secretion of weak organic acids including endogenous metabolites and drugs. Besides acidic metabolites of neurotransmitters, neuroactive tryptophan metabolites like kynurenate (KYNA), 3- hydroxykynurenine (3-HK) and quinolinate (QUIN) are postulated to be excreted via a probenecid-sensitive transport System. Immunohistochemical analyses in mouse kidneys revealed the localization of mouse OATl (mOAT1) in proximal convoluted tubules. Examinations of mouse brain sections showed positive stainings of cortical neurons. Additionally, we investigated the interaction of mOATl with several tryptophan metabolites. We transiently transfected COS 7 cells with mOAT1 and measured the influence of tryptophan metabolites on [~HIPAH u take 1mM of probenecid, KYNA, 3-HK, 5- hydroxyindoEte (i-HIAA) and anthranilate (ANTRA) decreased PAH uptake by 50-85%, demonstrating that all these compounds bind to OAT1. 1mM QUIN showed only a slight, but significant inhibition of PAH uptake. Preloading the cells with 1mM glutarate, KYNA, 3-HK, 5-HIAA, ANTRA or QUIN revealed a substantial trans-stimulation of PAH uptake by glutarate, and a significant effect for 5-HIAA, indicating that this compound is translocated by OAT1.
Subjects 0606 Physiology
1116 Medical Physiology
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Abstract number: P03-11

 
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Created: Wed, 03 Mar 2010, 10:05:49 EST by Laura McTaggart on behalf of Faculty of Science