Analysis of early nephron patterning reveals a role for distal RV proliferation in fusion to the ureteric tip via a cap mesenchyme-derived connecting segment

Georgas, Kylie, Rumballe, Bree, Valerius, M. Todd, Chiu, Han Sheng, Thiagarajan, Rathi D., Lesieur, Emmanuelle, Aronow, Bruce J., Brunskill, Eric W., Combes, Alexander N., Tang, Dave, Taylor, Darrin, Grimmond, Sean M., Potter, S. Steven, McMahon, Andrew P. and Little, Melissa H. (2009) Analysis of early nephron patterning reveals a role for distal RV proliferation in fusion to the ureteric tip via a cap mesenchyme-derived connecting segment. Developmental Biology, 332 2: 273-286. doi:10.1016/j.ydbio.2009.05.578


Author Georgas, Kylie
Rumballe, Bree
Valerius, M. Todd
Chiu, Han Sheng
Thiagarajan, Rathi D.
Lesieur, Emmanuelle
Aronow, Bruce J.
Brunskill, Eric W.
Combes, Alexander N.
Tang, Dave
Taylor, Darrin
Grimmond, Sean M.
Potter, S. Steven
McMahon, Andrew P.
Little, Melissa H.
Title Analysis of early nephron patterning reveals a role for distal RV proliferation in fusion to the ureteric tip via a cap mesenchyme-derived connecting segment
Journal name Developmental Biology   Check publisher's open access policy
ISSN 0012-1606
1095-564X
Publication date 2009-08-15
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.ydbio.2009.05.578
Volume 332
Issue 2
Start page 273
End page 286
Total pages 14
Editor R. Krumlauf
Place of publication San Diego, C.A., U.S.A.
Publisher Academic Press
Collection year 2010
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
060403 Developmental Genetics (incl. Sex Determination)
Abstract While nephron formation is known to be initiated by a mesenchyme-to-epithelial transition of the cap mesenchyme to form a renal vesicle (RV), the subsequent patterning of the nephron and fusion with the ureteric component of the kidney to form a patent contiguous uriniferous tubule has not been fully characterized. Using dual section in situ hybridization (SISH)/immunohistochemistry (IHC) we have revealed distinct distal/proximal patterning of Notch, BMP and Wnt pathway components within the RV stage nephron. Quantitation of mitoses and Cyclin D1 expression indicated that cell proliferation was higher in the distal RV, reflecting the differential developmental programs of the proximal and distal populations. A small number of RV genes were also expressed in the early connecting segment of the nephron. Dual ISH/IHC combined with serial section immunofluorescence and 3D reconstruction revealed that fusion occurs between the late RV and adjacent ureteric tip via a process that involves loss of the intervening ureteric epithelial basement membrane and insertion of cells expressing RV markers into the ureteric tip. Using Six2-eGFPCre × R26R-lacZ mice, we demonstrate that these cells are derived from the cap mesenchyme and not the ureteric epithelium. Hence, both nephron patterning and patency are evident at the late renal vesicle stage.
Keyword Renal vesicle
nephron development
gene expression
renal connecting tubule formation
nephron patterning
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Created: Mon, 01 Mar 2010, 10:44:37 EST by Susan Allen on behalf of Institute for Molecular Bioscience