4D microscopy

Mironov, Alexander A., Micaroni, Massimo and Beznoussenko, Galina V. (2008). 4D microscopy. In: EMC 2008 14th European Microscopy Congress: Instrumentation and Methods. 14th European Microscopy Congress (EMC), Aachen, Germany, (). 1-5 September 2008. doi:10.1007/978-3-540-85156-1_395

Author Mironov, Alexander A.
Micaroni, Massimo
Beznoussenko, Galina V.
Title of paper 4D microscopy
Conference name 14th European Microscopy Congress (EMC)
Conference location Aachen, Germany
Conference dates 1-5 September 2008
Proceedings title EMC 2008 14th European Microscopy Congress: Instrumentation and Methods
Place of Publication Berlin Heidelberg
Publisher Springer
Publication Year 2008
Sub-type Published abstract
DOI 10.1007/978-3-540-85156-1_395
ISBN 978-3-540-85154-7
Volume Volume 1
Language eng
Abstract/Summary The routine light microscopy provides only a 2D imaging. Using optical sectioning and consecutive 3D-reconstruction one gets 3D imaging. A 3D imaging in time (3D video microscopy) gives a 4D-imaging. Finally, combination of a 4D-imaging of two fluorochromes (usually CFP and YFP) determines a 5D-imaging. After fixation and staining of the same cell, addition of a red fluorochrome (the correlative light-light microscopy) will give a >5D-imaging. After production of 5D-movies one should fix cells at a define stage of the life cycle of an organelle just observed and then analyze the same organelle under a light or electron microscope using an additional immunolabeling, including FRET, and more precise 3D-reconstruction with an electron microscopic tomography of the very same organelle.
Subjects 06 Biological Sciences
Keyword 4D imaging
correlative microscopy
electron microscopic tomography
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status Unknown

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Created: Mon, 01 Mar 2010, 09:38:04 EST by Laura McTaggart on behalf of Institute for Molecular Bioscience