Development of retinal pigment epithelial cell culture on Bombyx mori silk fibroin (BMSF) membrane for retinal transplantation

Kwan, Anthony, Cheng, Sean, Barnard, Zeke, Zainuddin, Zainuddin, Harkin, Damien and Chirila, Traian (2007). Development of retinal pigment epithelial cell culture on Bombyx mori silk fibroin (BMSF) membrane for retinal transplantation. In: Clinical and Experimental Ophthalmology. Special Issue: 39th Annual Scientific Congress of The Royal Australian and New Zealand College of Ophthalmologists. 39th Annual Scientific Congress of The Royal Australian and New Zealand College of Ophthalmologists, Perth, Western Australia, Perth, WA, Australia, (A40-A40). 4-8 November 2007. doi:10.1111/j.1442-9071.2007.01595.x


Author Kwan, Anthony
Cheng, Sean
Barnard, Zeke
Zainuddin, Zainuddin
Harkin, Damien
Chirila, Traian
Title of paper Development of retinal pigment epithelial cell culture on Bombyx mori silk fibroin (BMSF) membrane for retinal transplantation
Conference name 39th Annual Scientific Congress of The Royal Australian and New Zealand College of Ophthalmologists, Perth, Western Australia
Conference location Perth, WA, Australia
Conference dates 4-8 November 2007
Proceedings title Clinical and Experimental Ophthalmology. Special Issue: 39th Annual Scientific Congress of The Royal Australian and New Zealand College of Ophthalmologists   Check publisher's open access policy
Place of Publication Melbourne, Australia
Publisher Wiley-Blackwell Publishing Asia
Publication Year 2007
DOI 10.1111/j.1442-9071.2007.01595.x
ISSN 1442-6404
1442-6404
Volume 35
Issue Supp.1
Start page A40
End page A40
Total pages 1
Language eng
Formatted Abstract/Summary
Purpose
One theoretical treatment of age-related macular degeneration is retinal pigment epithelial cell (RPE) transplantation. The ideal substrate for RPE culture and transplantation has yet been developed. Here we evaluate a novel substratum for RPE culture which is based on silk protein derived from domesticated silkworm Bombyx mori silk fibroin (BMSF) which has been used successfully as a substrate for human corneal limbal epithelial cells.

Method
Commercially available RPE cell line ARPE-19  Cells was seeded and cultured under standard culture conditions on plastic well and BMSF membrane for 72 hours. The cultures were fixed; nuclei, actin, and cell membrane were stained with Hoechst 33342, Rhodamine, and Phalloidin stains respectively. RPE cell attachment and growth on BMSF was compared to plastic wells. The experiment
was repeated with extracellular matrix proteins (ECM) (e.g. laminin, collagen type IV, fibronectin, and vitronectin) attached to BMSF.

Results
The ARPE-19 cell attachment rate on un-modified BMSF membrane is more than 40% of that achieved on plastic well surface. This rate is increased when BMSF has
been treated with ECM. Morphology of ARPE-19 cells on BSMF is similar to those on plastic wells.

Conclusion
MSF appears to facilitate mono-layer RPE formation with normal morphology. The cell attachment is reduced when compared to standard plastic wells, but it appears to increase when ECM are added to BMSF. Silk membrane appears to be a potential substrate for RPE culture. Further studies are required to assess the safety and feasibility of BMSF as a substrate for RPE transplantation.
Subjects 1113 Ophthalmology and Optometry
Keyword Retinal pigment epithelial cell (RPE)
RPE culture
Bombyx mori silk fibroin (BMSF)
Macular degeneration
RPE transplantation
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status Unknown

 
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Created: Thu, 25 Feb 2010, 14:52:19 EST by Gerald Martin on behalf of Faculty Of Health Sciences