Genotyping methodology and frequencies of the myostatin F94L mutation in Australian cattle breeds

Vankan, Dianne M., Corley, Sean, Wayne, Deanne and Fortes, Marina (2008). Genotyping methodology and frequencies of the myostatin F94L mutation in Australian cattle breeds. In: Proceedings of the 31st Internationl Conference on Animal Genetics. 31st Conference of the International Society of Animal Genetics, Amsterdam, Netherlands, (). July 20-24, 2008.


Author Vankan, Dianne M.
Corley, Sean
Wayne, Deanne
Fortes, Marina
Title of paper Genotyping methodology and frequencies of the myostatin F94L mutation in Australian cattle breeds
Conference name 31st Conference of the International Society of Animal Genetics
Conference location Amsterdam, Netherlands
Conference dates July 20-24, 2008
Proceedings title Proceedings of the 31st Internationl Conference on Animal Genetics
Publication Year 2008
Sub-type Poster
Total pages 1
Language eng
Formatted Abstract/Summary
The F94L SNP (g.433C>A) in the growth differentiation factor 8 (GDF8) gene, commonly known as the myostatin (MSTN) gene, has recently been shown to be functionally associated with increased muscle mass and carcass yield in cattle. Since the F94L mutation is not, like other MSTN mutations, associated with reduced fertility and dystocia, it is a candidate for introgression into other breeds to improve retail beef yield, and development of an accurate test for genotyping this mutation is warranted. Accurate genotyping of the F94L SNP by primer extension is not possible so genotyping has been achieved using PCR-RFLP. We found that variations in the efficiency of cleavage of PCR amplicons by Taq1 compromised the accuracy of genotyping, resulting in an overestimation of the frequency of the mutant A allele. We developed two real-time PCR assays that both accurately genotype the F94L mutation in cattle based on allele-specific and high resolution melt analysis. The frequency of the F94L mutation was determined in 960 animals from 14 breeds of cattle. The mutation was present in Simmental (0.8%), Piedmontese (2%), Droughtmaster (4%) and Limousin (94.2%) but not found in Salers, Angus, Poll Hereford, Hereford, Gelbvieh, Charolais, Jersey, Brahman, Holstein and Maine Anjou.
Subjects 0799 Other Agricultural and Veterinary Sciences
0604 Genetics
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Category 2000: Markers and selection - Gene expression and mapping. Poster #2144.

 
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Created: Thu, 25 Feb 2010, 11:35:22 EST by Elissa Saffery on behalf of Faculty Of Nat Resources, Agric & Veterinary Sc