Distribution of the Ca2+-binding S100A1 protein at different sarcomere lengths of slow and fast rat skeletal muscles

Maco, B., Uhrik, B and Heizmann, C. W. (2000) Distribution of the Ca2+-binding S100A1 protein at different sarcomere lengths of slow and fast rat skeletal muscles. General Physiology and Biophysics, 19 2: 237-244.


Author Maco, B.
Uhrik, B
Heizmann, C. W.
Title Distribution of the Ca2+-binding S100A1 protein at different sarcomere lengths of slow and fast rat skeletal muscles
Journal name General Physiology and Biophysics   Check publisher's open access policy
ISSN 0231-5882
Publication date 2000-06
Sub-type Article (original research)
Volume 19
Issue 2
Start page 237
End page 244
Total pages 8
Place of publication Slovakia
Publisher Slovenska Akademia Vied, Ustav Molekularnej Fyziologie a Genetiky
Language eng
Subject 06 Biological Sciences
Abstract The localization of S100A1 in rat soleus (SOL) and extensor digitorum longus (EDL) muscles was studied immunocytochemically at different sarcomere lengths (stretched, relaxed and contracted) at the ultrastructural level. The muscle fibres were contracted by application of 15 mmol/l caffeine. Following aldehyde fixation, dehydration and embedding in Lowicryl HM20 (-35 degrees C) ultrathin sections were incubated with rabbit polyclonal antiserum against S100A1. Goat antirabbit secondary antibodies conjugated with 10 nm gold particles were used to visualize antigen sites. Relative areas of Z-lines, A- and I-bands were estimated from longitudinal sections by the point counting method. The highest densities of the particles were found at the Z-lines. A higher incidence of S100A1 antigen sites in I-bands than in A-bands and a higher density of S100A1 in lateral parts of A-bands (with actin and myosin filaments overlapping) compared with the central area of A-bands are consistent with an interaction of S100A1 with F-actin in skeletal muscles. Antigen sites were also present at M-lines and at distinct locations of the sarcoplasmic reticulum.
Keyword Ca2+-binding proteins
S100A1
F-actin
myofibrils
muscle cells
sarcoplasmic reticulum
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Wed, 24 Feb 2010, 08:57:28 EST by Laura McTaggart on behalf of Faculty of Science