Ocular nanoparticle toxicity and transfection of the retina and retinal pigment epithelium

Prow, Tarl W., Bhutto, Imran, Kim, Sahng Y., Greebe, Rhonda, Merges, Carol, McLeod, Scott, Uno, Koichi, Mennon, Mohamed, Rodriguez, Li, Leong, Kam and Lutty, Gerard A. (2008) Ocular nanoparticle toxicity and transfection of the retina and retinal pigment epithelium. Nanomedicine: Nanotechnology, Biology and Medicine, 4 4: 340-349. doi:10.1016/j.nano.2008.06.003


Author Prow, Tarl W.
Bhutto, Imran
Kim, Sahng Y.
Greebe, Rhonda
Merges, Carol
McLeod, Scott
Uno, Koichi
Mennon, Mohamed
Rodriguez, Li
Leong, Kam
Lutty, Gerard A.
Title Ocular nanoparticle toxicity and transfection of the retina and retinal pigment epithelium
Journal name Nanomedicine: Nanotechnology, Biology and Medicine   Check publisher's open access policy
ISSN 1549-9634
1549-9642
Publication date 2008-12
Sub-type Article (original research)
DOI 10.1016/j.nano.2008.06.003
Volume 4
Issue 4
Start page 340
End page 349
Total pages 10
Place of publication Philadelphia, PA, U.S.A.
Publisher Elsevier
Language eng
Subject 1007 Nanotechnology
0304 Medicinal and Biomolecular Chemistry
Abstract Chitosan, PCEP (poly{[(cholesteryl oxocarbonylamido ethyl) methyl bis(ethylene) ammonium iodide] ethyl phosphate}), and magnetic nanoparticles (MNPs) were evaluated for the safe delivery of genes in the eye. Rabbits were injected with nanoparticles either intravitreally (IV) or subretinally (SR) and sacrificed 7 days later. Eyes were grossly evaluated for retinal pigment epithelium abnormalities, retinal degeneration, and inflammation. All eyes were cryopreserved and sectioned for analysis of toxicity and expression of either enhanced green or red fluorescent proteins. All of the nanoparticles were able to transfect cells in vitro and in vivo. IV chitosan showed inflammation in 12/13 eyes, whereas IV PCEP and IV MNPs were not inflammatory and did not induce retinal pathology. SR PCEP was nontoxic in the majority of cases but yielded poor transfection, whereas SR MNPs were nontoxic and yielded good transfection. Therefore, we conclude that the best nanoparticle evaluated in vivo was the least toxic nanoparticle tested, the MNP.
Keyword Chitosan
Magnetic nanoparticle
Gene delivery
Retina
Toxicity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 44 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 52 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 23 Feb 2010, 12:22:43 EST by Michael Affleck on behalf of Faculty Of Health Sciences