Autoantibody profiling to identify biomarkers of key pathogenic pathways in mucinous ovarian cancer

Tang, Liangdan, Yang, Junzheng, Ng, Shu-Kay, Rodriguez, Noah, Choi, Pui-Wah, Vitonis, Allison, Wang, Kui, McLachlan, Geoffrey J., Caiazzo, Robert J., Liu, Brian C.-S., Welch, Brian C.-S., Cramer, Daniel W., Berkowitz, Ross S. and Ng, Shu-Wing (2010) Autoantibody profiling to identify biomarkers of key pathogenic pathways in mucinous ovarian cancer. European Journal of Cancer, 46 1: 170-179.


Author Tang, Liangdan
Yang, Junzheng
Ng, Shu-Kay
Rodriguez, Noah
Choi, Pui-Wah
Vitonis, Allison
Wang, Kui
McLachlan, Geoffrey J.
Caiazzo, Robert J.
Liu, Brian C.-S.
Welch, Brian C.-S.
Cramer, Daniel W.
Berkowitz, Ross S.
Ng, Shu-Wing
Title Autoantibody profiling to identify biomarkers of key pathogenic pathways in mucinous ovarian cancer
Journal name European Journal of Cancer  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2010-01
Sub-type Article
Year available 2009
DOI 10.1016/j.ejca.2009.10.003
Volume number 46
Issue number 1
ISSN 0959-8049
1879-0852
1359-6349
Start page 170
End page 179
Total pages 10
Editor John Smyth
Place of publication Oxford, U.K
Publisher Pergamon Press.
Collection year 2011
Language eng
Formatted abstract Mucinous epithelial ovarian cancers are clinically and morphologically distinct from the other histopathologic subtypes of ovarian cancer. Unlike other ovarian subtypes, epidemiologic studies have indicated that tobacco exposure is a significant risk factor for developing mucinous ovarian cancer. Detection of autoantibody reactivity is useful in biomarker discovery and for explaining the role of important pathophysiologic pathways in disease. In order to study if there are specific antibody biomarkers in the plasma samples of mucinous ovarian cancer patients, we have initiated a screen by employing a 'reverse capture antibody microarray' platform that uses native host antigens derived from mucinous ovarian tissues as 'baits' for the capture of differentially labelled patient and control autoantibodies. Thirty-five autoantibodies that were significantly elevated in the cancer plasma samples compared with healthy controls, and six autoantibodies that segregated smoking and non-smoking patients were identified. Functional annotation of the antibody targets has identified nine target antigens involved in integrin and Wnt signalling pathways. Immunohistochemistry of archived ovarian specimens showed significant overexpression of eight of the nine target antigens in mucinous ovarian tumour tissues, suggesting that plasma autoantibodies from mucinous ovarian cancer patients might have heightened reactivities with epitopes presented by these overexpressed antigens. Autoantibody profiling may have an unexpected utility in uncovering key signalling pathways that are dysregulated in the system of interest.
© 2009 Elsevier Ltd. All rights reserved.
Keyword Autoantibody
Ovarian cancer
Smoking
Profiling
Signalling pathway
K-ras protooncogene
Cigarette-smoking
Protein microarrays
Immune-response
Beta-catenin
Lung-cancer
Identification
Expression
Carcinoma
Antigen
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 16 November 2009.

Document type: Journal Article
Sub-type: Article
Collections: School of Mathematics and Physics
Official 2011 Collection
 
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Created: Sun, 21 Feb 2010, 00:08:16 EST