Toll-like receptor 7 function is reduced in adolescents with asthma

Roponen, M., Yerkovich, S. T., Hollams, E., Sly, P. D., Holt, P. G. and Upham, J. W. (2010) Toll-like receptor 7 function is reduced in adolescents with asthma. European Respiratory Journal, 35 1: 64-71. doi:10.1183/09031936.00172008

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Author Roponen, M.
Yerkovich, S. T.
Hollams, E.
Sly, P. D.
Holt, P. G.
Upham, J. W.
Title Toll-like receptor 7 function is reduced in adolescents with asthma
Journal name European Respiratory Journal   Check publisher's open access policy
ISSN 0106-4339
0904-1850
1399-3003
Publication date 2010-01-01
Year available 2009
Sub-type Article (original research)
DOI 10.1183/09031936.00172008
Open Access Status
Volume 35
Issue 1
Start page 64
End page 71
Total pages 8
Editor Anh Tuan Dinh-Xuan
Vito Brusasco
Place of publication Copenhagen, Denmark
Publisher Munksgaard
Collection year 2011
Language eng
Formatted abstract
Anti-viral innate immune responses may be impaired in asthma, although the mechanisms are not well understood. Toll-like receptors (TLRs) 7 and 3 are particularly relevant for initiating responses to common respiratory viruses, as they recognise single-stranded viral RNA and double-stranded viral RNA, respectively. The aim of the present study was to investigate TLR7 and TLR3 function in 14-yr-old adolescents with asthma. Blood mononuclear cells obtained from 17 atopic asthmatics, 29 atopic, non-asthmatics and 21 healthy, non-atopic individuals, were stimulated with the TLR7 agonist imiquimod and the TLR3 agonist poly I:C. Expression of anti-viral molecules was measured by real-time PCR. Concentrations of interferon-c-inducible cytokine protein (IP)-10 and interleukin (IL)-6 were measured by ELISA. TLR7-induced myxovirus resistance protein A and 2′5′ oligoadenylate synthetase mRNA expression and protein levels of IP-10 were significantly lower in asthma subjects compared with healthy subjects (p=0.041, p=0.003 and p=0.001 respectively). There was a significant negative correlation between total serum immunoglobulin E and IP-10 following TLR7 stimulation. However, TLR3-induced responses did not vary with asthma or atopy. IL-10 mRNA and IL-6 protein synthesis were similar in asthmatic and control subjects. In conclusion, TLR7 function is reduced in adolescents with asthma and this may contribute to susceptibility to respiratory viral infections. Copyright
©ERS Journals Ltd 2010.
Keyword Asthma
Innate immunity
Toll-like receptor
Virus
Respiratory infections
Epithelial cells
Rhinovirus
Exacerbations
Responses
Children
Allergen
TLR7
Association
Activation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online before print July 30, 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Medicine Publications
 
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Created: Sun, 21 Feb 2010, 00:08:00 EST