The REDUCED LEAFLET Genes Encode Key Components of the trans-Acting Small Interfering RNA Pathway and Regulate Compound Leaf and Flower Development in Lotus japonicus

Yan, Jun, Cai, Xuefei, Luo, Jianghong, Sato, Shusei, Jiang, Qunyi, Yang, Jun, Cao, Xiangling, Hu, Xiaohe, Tabata, Satoshi, Gresshoff, Peter M. and Luo, Da (2010) The REDUCED LEAFLET Genes Encode Key Components of the trans-Acting Small Interfering RNA Pathway and Regulate Compound Leaf and Flower Development in Lotus japonicus. Plant Physiology, 152 2: 797-807. doi:10.1104/pp.109.140947


Author Yan, Jun
Cai, Xuefei
Luo, Jianghong
Sato, Shusei
Jiang, Qunyi
Yang, Jun
Cao, Xiangling
Hu, Xiaohe
Tabata, Satoshi
Gresshoff, Peter M.
Luo, Da
Title The REDUCED LEAFLET Genes Encode Key Components of the trans-Acting Small Interfering RNA Pathway and Regulate Compound Leaf and Flower Development in Lotus japonicus
Formatted title
The REDUCED LEAFLET genes encode key components of the trans-acting siRNA pathway and regulate compous leaf and flower development in Lotus japonicus
Journal name Plant Physiology   Check publisher's open access policy
ISSN 0032-0889
Publication date 2010-02
Year available 2009
Sub-type Article (original research)
DOI 10.1104/pp.109.140947
Volume 152
Issue 2
Start page 797
End page 807
Total pages 11
Editor Donald R Ort
Place of publication United States
Publisher American Society of Plant Biologists
Collection year 2010
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
829899 Environmentally Sustainable Plant Production not elsewhere classified
060111 Signal Transduction
060703 Plant Developmental and Reproductive Biology
060407 Genome Structure and Regulation
Abstract The endogenous trans-acting small interfering RNA (ta-siRNA) pathway plays a conserved role in adaxial-abaxial patterning of lateral organs in simple-leafed plant species. However, its function in compound-leafed species is largely unknown. Using the compound-leafed species Lotus japonicus, we identified and characterized two independent mutants, reduced leaflet1 (rel1) and rel3, whose most conspicuous defects in compound leaves are abaxialized leaflets and reduction in leaflet number. Concurrent mutations in REL genes also compromise flower development and result in radial symmetric floral organs. Positional cloning revealed that REL1 and REL3 encode the homologs of Arabidopsis (Arabidopsis thaliana) SUPPRESSOR OF GENE SILENCING3 and ARGONAUTE7/ZIPPY, respectively, which are key components of the ta-siRNA pathway. These observations, together with the expression and functional data, demonstrated that the ta-siRNA pathway plays conserved yet distinct roles in the control of compound leaf and flower development in L. japonicus. Moreover, the phenotypic alterations of lateral organs in ta-siRNA-deficient mutants and the regulation of downstream targets by the ta-siRNA pathway in L. japonicus were similar to those in the monocots but different from Arabidopsis, indicating many parallels between L. japonicus and the monocots in the control of lateral organ development by the ta-siRNA pathway.
Formatted abstract
The endogenous trans-acting small interfering RNA (ta-siRNA) pathway plays a conserved role in adaxial-abaxial patterning of lateral organs in simple-leafed plant species. However, its function in compound-leafed species is largely unknown. Using the compound-leafed species Lotus japonicus, we identified and characterized two independent mutants, reduced leaflet1 (rel1) and rel3, whose most conspicuous defects in compound leaves are abaxialized leaflets and reduction in leaflet number. Concurrent mutations in REL genes also compromise flower development and result in radial symmetric floral organs. Positional cloning revealed that REL1 and REL3 encode the homologs of Arabidopsis (Arabidopsis thaliana) SUPPRESSOR OF GENE SILENCING3 and ARGONAUTE7/ZIPPY, respectively, which are key components of the ta-siRNA pathway. These observations, together with the expression and functional data, demonstrated that the ta-siRNA pathway plays conserved yet distinct roles in the control of compound leaf and flower development in L. japonicus. Moreover, the phenotypic alterations of lateral organs in ta-siRNA-deficient mutants and the regulation of downstream targets by the ta-siRNA pathway in L. japonicus were similar to those in the monocots but different from Arabidopsis, indicating many parallels between L. japonicus and the monocots in the control of lateral organ development by the ta-siRNA pathway.
Keyword Shoot apical meristem
Lateral organs
Arabidopsis thaliana
siRNA pathway
Cell fate
Expression
Polarity
PHANTASTICA
KANADI
Rice
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
2010 Higher Education Research Data Collection
 
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Created: Sun, 21 Feb 2010, 00:01:44 EST