Liver fatty acid-binding protein gene-ablated female mice exhibit increased age-dependent obesity1–3

Martin, Gregory G., Atshaves, Barbara P., McIntosh, Avery L., Mackie, John T., Kier, Ann B. and Schroeder, Friedhelm (2008) Liver fatty acid-binding protein gene-ablated female mice exhibit increased age-dependent obesity1–3. Journal of Nutrition, 138 10: 1859-1865.


Author Martin, Gregory G.
Atshaves, Barbara P.
McIntosh, Avery L.
Mackie, John T.
Kier, Ann B.
Schroeder, Friedhelm
Title Liver fatty acid-binding protein gene-ablated female mice exhibit increased age-dependent obesity1–3
Formatted title
Liver fatty acid-binding protein gene-ablated female mice exhibit increased age-dependent obesity1–3
Journal name Journal of Nutrition   Check publisher's open access policy
ISSN 0022-3166
Publication date 2008-10-01
Sub-type Article (original research)
Volume 138
Issue 10
Start page 1859
End page 1865
Total pages 7
Place of publication Bethesda, U.S.
Publisher American Society for Nutrition
Language eng
Subject 0707 Veterinary Sciences
Formatted abstract
Previous work conducted in our laboratory suggested a role for liver fatty acid-binding protein (L-FABP) in obesity that develops in aging female L-FABP gene-ablated (–/–) mice. To examine this possibility in more detail, cohorts of wild-type (+/+) and L-FABP (–/–) female mice were fed a standard, low-fat, nonpurified rodent diet for up to 18 mo. Various obesity-related parameters were examined, including body weight and fat and lean tissue mass. Obesity in (–/–) mice was associated with increased expression of nuclear receptors that induce PPAR (e.g. hepatocyte nuclear factor 1, genotype effect) and of PPAR-regulated proteins involved in uptake of free (lipoprotein lipase and fatty acid transport protein, genotype, and/or age effect) and esterified (scavenger receptor class B type 1, genotype effect) long-chain fatty acids (LCFA). Hepatic total lipid and neutral lipid levels were not affected by age or genotype, consistent with absence of gross and histologic steatosis. There was increased mRNA expression of liver proteins involved in LCFA oxidation [mitochondrial 3-oxoacyl-CoA thiolase (genotype effect) and butyryl-CoA dehydrogenase (genotype and/or age effect)], increased expression of LCFA esterification enzymes [glycerol-3-phosphate acyltransferase (age x genotype effect) and acyl-CoA:cholesterol acyltransferase-2 (genotype and/or age effect)], and increased expression of proteins involved in intracellular transfer and secretion of esterified LCFA [liver microsomal triacylglycerol transfer protein (genotype effect), serum apolipoprotein (apo) B (genotype or age effect), and liver apoB (age and age x genotype effect)]. The data support a working model in which obesity development in these mice results from shifts toward reduced energy expenditure and/or more efficient energy uptake in the gut. © 2008 American Society for Nutrition.
Keyword Aging/physiology
Animals
Fatty Acid-Binding Proteins/deficiency
Fatty Acid-Binding Proteins/genetics
Female
Gene Expression Regulation, Developmental
Lipids/physiology
Liver/physiology
Mice
Mice, Inbred C57BL
Mice, Knockout
Obesity/genetics
Proteins/genetics
RNA/genetics
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Veterinary Science Publications
 
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Created: Sat, 20 Feb 2010, 01:27:51 EST by Tara Johnson on behalf of Faculty Of Nat Resources, Agric & Veterinary Sc