Golgi enzymes are enriched in perforated zones of Golgi cisternae but are depleted in COPI vesicles

Kweon, Hee-Seok, Beznoussenko, Galina V., Micaroni, Massimo, Polishchuk, Roman S., Trucco, Alvar, Martella , Oliviano, Di Giandomenico, Daniele, Marra, Pierfrancesco, Fusella, Aurora, Di Pentima, Alessio, Berger, Eric G., Geerts, Willie J. C., Koster, Abraham J., Burger, Koert N. J., Luini , Alberto and Mironov, Alexander A. (2004) Golgi enzymes are enriched in perforated zones of Golgi cisternae but are depleted in COPI vesicles. Molecular biology of the cell, 15 10: 4710-4724. doi:10.1091/mbc.E03-12-0881

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Author Kweon, Hee-Seok
Beznoussenko, Galina V.
Micaroni, Massimo
Polishchuk, Roman S.
Trucco, Alvar
Martella , Oliviano
Di Giandomenico, Daniele
Marra, Pierfrancesco
Fusella, Aurora
Di Pentima, Alessio
Berger, Eric G.
Geerts, Willie J. C.
Koster, Abraham J.
Burger, Koert N. J.
Luini , Alberto
Mironov, Alexander A.
Title Golgi enzymes are enriched in perforated zones of Golgi cisternae but are depleted in COPI vesicles
Journal name Molecular biology of the cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2004-10
Sub-type Article (original research)
DOI 10.1091/mbc.E03-12-0881
Open Access Status File (Publisher version)
Volume 15
Issue 10
Start page 4710
End page 4724
Total pages 5
Editor Keith Mostov
Place of publication Bethesda, Maryland, U.S.A.
Publisher American Society for Cell Biology
Language eng
Subject 0601 Biochemistry and Cell Biology
1112 Oncology and Carcinogenesis
111201 Cancer Cell Biology
Abstract In the most widely accepted version of the cisternal maturation/progression model of intra-Golgi transport, the polarity of the Golgi complex is maintained by retrograde transport of Golgi enzymes in COPI-coated vesicles. By analyzing enzyme localization in relation to the three-dimensional ultrastructure of the Golgi complex, we now observe that Golgi enzymes are depleted in COPI-coated buds and 50- to 60-nm COPI-dependent vesicles in a variety of different cell types. Instead, we find that Golgi enzymes are concentrated in the perforated zones of cisternal rims both in vivo and in a cell-free system. This lateral segregation of Golgi enzymes is detectable in some stacks during steady-state transport, but it was significantly prominent after blocking endoplasmic reticulum-to-Golgi transport. Delivery of transport carriers to the Golgi after the release of a transport block leads to a diminution in Golgi enzyme concentrations in perforated zones of cisternae. The exclusion of Golgi enzymes from COPI vesicles and their transport-dependent accumulation in perforated zones argues against the current vesicle-mediated version of the cisternal maturation/progression model.
Keyword Golgi
enzymes
COPI
cisternal rims
transport carriers
cisternae
vesicles
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 19 Feb 2010, 09:56:15 EST by Maria Campbell on behalf of Institute for Molecular Bioscience