Studying mechanosensitive ion channels using liposomes

Martinac, Boris, Rohde, Paul R., Battle, Andrew R., Petrov, Evgeny, Pal, Prithwish, Foo, Alexander Fook Weng, Vasquez, Valeria, Huynh, Thuan and Kloda, Anna (2010). Studying mechanosensitive ion channels using liposomes. In Volkmar Weissig (Ed.), Liposomes: Methods and Protocols Volume 2: Biological Membrane Models (pp. 31-54) New York, United states: Humana Press. doi:10.1007/978-1-60761-447-0_4

Author Martinac, Boris
Rohde, Paul R.
Battle, Andrew R.
Petrov, Evgeny
Pal, Prithwish
Foo, Alexander Fook Weng
Vasquez, Valeria
Huynh, Thuan
Kloda, Anna
Title of chapter Studying mechanosensitive ion channels using liposomes
Title of book Liposomes: Methods and Protocols Volume 2: Biological Membrane Models
Place of Publication New York, United states
Publisher Humana Press
Publication Year 2010
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-60761-447-0_4
Year available 2009
Series Methods in Molecular Biology
ISBN 9781607614463
ISSN 1064-3745
Editor Volkmar Weissig
Volume number 606
Chapter number 4
Start page 31
End page 54
Total pages 24
Total chapters 36
Collection year 2010
Language eng
Subjects 920111 Nervous System and Disorders
0601 Biochemistry and Cell Biology
Abstract/Summary Mechanosensitive (MS) ion channels are the primary molecular transducers of mechanical force into electrical and/or chemical intracellular signals in living cells. They have been implicated in innumerable mechanosensory physiological processes including touch and pain sensation, hearing, blood pressure control, micturition, cell volume regulation, tissue growth, or cellular turgor control. Much of what we know about the basic physical principles underlying the conversion of mechanical force acting upon membranes of living cells into conformational changes of MS channels comes from studies of MS channels reconstituted into artificial liposomes. Using bacterial MS channels as a model, we have shown by reconstituting these channels into liposomes that there is a close relationship between the physico-chemical properties of the lipid bilayer and structural dynamics bringing about the function of these channels.
Keyword MscL
liposome reconstitution
patch clamp
Epr Spectroscopy
FRET spectroscopy
Confocal Microscopy
Q-Index Code B1
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Tue, 16 Feb 2010, 11:30:15 EST by Bacsweet Kaur on behalf of School of Biomedical Sciences