Rapid access to omega conotoxin chimeras using native chemical ligation

Hopping , Gene, Lewis, Richard J. and Alewood, Paul F. (2009) Rapid access to omega conotoxin chimeras using native chemical ligation. Austalian Journal of Chemistry, 62 10: 1333-1338. doi:10.1071/CH09216


Author Hopping , Gene
Lewis, Richard J.
Alewood, Paul F.
Title Rapid access to omega conotoxin chimeras using native chemical ligation
Formatted title
Rapid Access to ω-Conotoxin Chimeras using Native Chemical Ligation



Journal name Austalian Journal of Chemistry   Check publisher's open access policy
ISSN 0004-9425
Publication date 2009-10
Year available 2009
Sub-type Article (original research)
DOI 10.1071/CH09216
Volume 62
Issue 10
Start page 1333
End page 1338
Total pages 6
Place of publication Collingwood, Vic., Austalia
Publisher C S I R O Publishing
Collection year 2010
Language eng
Subject C1
970103 Expanding Knowledge in the Chemical Sciences
03 Chemical Sciences
0304 Medicinal and Biomolecular Chemistry
Abstract Grafting different regions of related peptides together to form a single protein chimera is a valuable tool in rapidly elucidating regions of activity or selectivity in peptides and proteins. To conveniently evaluate the contributions of the N- and C-terminal segments of ω-conotoxins CVID and MVIIC to activity, we employed native chemical ligation in CVID-MVIIC chimera design. Assembly of these peptide segments via the ligation method improved overall yield and coupling efficiency, with no difficult sequences encountered in contrast to the traditional full-length chain assembly of CVID. Radio-ligand binding assays revealed regions of importance for receptor recognition.
Keyword Chain assemblies
Conotoxins
Coupling efficiency
Difficult sequencen
Ligand Binding
Native chemical ligation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 12 Feb 2010, 11:45:18 EST by Susan Allen on behalf of Institute for Molecular Bioscience