Intrastromal keratotomy with femtosecond laser avoids profibrotic TGF-beta1 induction

Meltendorf, Christian, Burbach, Guido J., Ohrloff, Christian, Ghebremedhin, Estifanos and Deller, Thomas (2009) Intrastromal keratotomy with femtosecond laser avoids profibrotic TGF-beta1 induction. Investigative Ophthalmology and Visual Science, 50 8: 3688-3695. doi:10.1167/iovs.08-2699

Author Meltendorf, Christian
Burbach, Guido J.
Ohrloff, Christian
Ghebremedhin, Estifanos
Deller, Thomas
Title Intrastromal keratotomy with femtosecond laser avoids profibrotic TGF-beta1 induction
Formatted title
Intrastromal Keratotomy with Femtosecond Laser Avoids Profibrotic TGF-β1 Induction
Journal name Investigative Ophthalmology and Visual Science   Check publisher's open access policy
ISSN 0146-0404
Publication date 2009-04
Sub-type Article (original research)
DOI 10.1167/iovs.08-2699
Volume 50
Issue 8
Start page 3688
End page 3695
Total pages 8
Place of publication Rockville, MD, United States
Publisher Association for Research in Vision and Ophthalmology
Collection year 2010
Language eng
Subject C1
920107 Hearing, Vision, Speech and Their Disorders
060603 Animal Physiology - Systems
Formatted abstract
To examine expression of the profibrotic cytokine TGF-beta1 after selective intrastromal corneal injury with the use of a femtosecond laser.
METHODS: Rabbits underwent monocular intrastromal keratotomy at a preoperatively determined corneal depth of 160 to 200 mum with the use of a femtosecond laser. Femtosecond laser-induced TGF-beta1 expression was compared in nonoperated control eyes and eyes treated with photorefractive keratectomy (PRK). Follow-up examinations were performed 1, 3, 7, and 28 days after surgery. TGF-beta1 protein was identified by immunofluorescence labeling. With the use of laser-capture microdissection, epithelial, stromal, and endothelial cell layers were collected, and changes in TGF-beta1 mRNA expression were quantified with quantitative RT-PCR. RESULTS: TGF-beta1 mRNA and protein expression did not significantly increase after intrastromal femtosecond laser keratotomy. In contrast, TGF-beta1 was induced in corneal epithelial and stromal cells after PRK and showed up to 23-fold higher TGF-beta1 mRNA levels compared with control corneas. The increase of TGF-beta1 mRNA levels after PRK was accompanied by increased TGF-beta1 protein production.
CONCLUSIONS: Isolated stromal injury with a femtosecond laser does not result in induction of the profibrotic cytokine TGF-beta1. Because TGF-beta1 has been implicated in a fibrotic response of the corneal stroma to injury, absence of TGF-beta1 induction argues for a favorable wound-healing response. These findings support highly selective intrastromal procedures in refractive surgery.
Keyword Animals
Corneal Stroma/ metabolism/ surgery
Endothelium, Corneal/metabolism
Epithelium, Corneal/metabolism
Fluorescent Antibody Technique, Indirect
Gene Expression Regulation/ physiology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: ERA 2012 Admin Only
School of Biomedical Sciences Publications
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Created: Thu, 11 Feb 2010, 10:29:47 EST by Bacsweet Kaur on behalf of School of Biomedical Sciences