Quantitative considerations for suspension array assays

Connolly, A.R., Palanisamy, R. and Trau, M. (2010) Quantitative considerations for suspension array assays. Journal of Biotechnology, 145 1: 17-22. doi:10.1016/j.jbiotec.2009.10.004


Author Connolly, A.R.
Palanisamy, R.
Trau, M.
Title Quantitative considerations for suspension array assays
Journal name Journal of Biotechnology   Check publisher's open access policy
ISSN 0168-1656
Publication date 2010-01-01
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.jbiotec.2009.10.004
Volume 145
Issue 1
Start page 17
End page 22
Total pages 6
Editor A Puehler
Place of publication Netherlands
Publisher Elsevier B V
Collection year 2010
Language eng
Subject C1
0601 Biochemistry and Cell Biology
Formatted abstract
A novel format of a multiplex microbead assay that is capable of accurately and reproducibly measuring small changes in 10(8) copies of a nucleic acid is reported herein. The dynamic range of the assay was 32-fold greater than conventional multiplex microbead assays. The assay was modeled mathematically which enabled the accuracy of DNA quantification to be formally analysed using a logical theoretical framework. The assay was also evaluated on RNA extracts taken from stimulated macrophages, to demonstrate that changes in gene expression can be accurately monitored in a biological system. We believe the reproducibility and accuracy of the assay presented herein will be useful for high-throughput, multiplex sensitive detection of nucleic acids over a large dynamic range in a research or diagnostic setting. Crown Copyright © 2009 Published by Elsevier B.V. All rights reserved.
Keyword Suspension arrays
DNA hybridisation
DNA quantification
Microbeads
Flow cytometry
Immobilised oligonucleotides
Gene-expression Patterns
Isoparametric analysis
Signal amplification
Microarray
Macrophages
Dna
Hybridization
Microspheres
Performance
Technology
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
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Created: Sun, 07 Feb 2010, 00:04:44 EST