Characterization of microRNAs encoded by the bovine herpesvirus 1 genome

Glazov, Evgeny A., Horwood, Paul F., Assavalapsakul, Wanchai, Kongsuwan, Kritaya, Mitchell, Roger W., Mitter, Neena and Mahony, Timothy J. (2010) Characterization of microRNAs encoded by the bovine herpesvirus 1 genome. Journal of General Virology, 91 1: 32-41. doi:10.1099/vir.0.014290-0


Author Glazov, Evgeny A.
Horwood, Paul F.
Assavalapsakul, Wanchai
Kongsuwan, Kritaya
Mitchell, Roger W.
Mitter, Neena
Mahony, Timothy J.
Title Characterization of microRNAs encoded by the bovine herpesvirus 1 genome
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2010-01
Year available 2009
Sub-type Article (original research)
DOI 10.1099/vir.0.014290-0
Volume 91
Issue 1
Start page 32
End page 41
Total pages 10
Place of publication Reading, Berks, United Kingdom
Publisher Society for General Microbiolgoy
Collection year 2011
Language eng
Abstract Bovine herpesvirus 1 (BoHV-1) is a ubiquitous and important pathogen of cattle worldwide. This study reports the identification of 10 microRNA (miRNA) genes, Bhv1-mir-B1–Bhv1-mir-B10, encoded by the BoHV-1 genome that were processed into 12 detectable mature miRNAs as determined by ultra-high throughput sequencing bioinformatics analyses of small RNA libraries and expression studies. We found that four of the miRNA genes were present as two copies in the BoHV-1 genome, resulting in a total of 14 miRNA encoding loci. Unique features of the BoHV-1 miRNAs include evidence of bidirectional transcription and a close association of two miRNA genes with the origin of replication, including one miRNA that is encoded within the origin of replication. The miRNA gene Bhv1-mir-B5 was encoded on the opposite DNA strand to the latency associated transcript, potentially giving rise to antisense transcripts originating from this locus. The association of herpesvirus miRNAs with latency appears to be a common feature in the alphaherpesviruses. Analyses of the BoHV-5 genome for putative miRNA gene orthologues identified a high degree of evolutionary conservation for nine of the BoHV-1 miRNA genes. The possible roles for BoHV-1 miRNAs in the regulation of known BoHV-1 transcription units and the genetics of the BoHV-1 genotypes are also discussed.
Keyword Herpesvirus 1
Genes
Molecular sequence data
RNA analysis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Sun, 07 Feb 2010, 00:04:18 EST