Haemolytic fungi isolated from sago starch in Papua New Guinea

Greenhill, A. R., Blaney, B. J., Shipton, W. A., Pue, A., Fletcher, M. T. and Warner, J. M. (2010) Haemolytic fungi isolated from sago starch in Papua New Guinea. MYCOPATHOLOGIA, 169 2: 107-115. doi:10.1007/s11046-009-9235-3

Author Greenhill, A. R.
Blaney, B. J.
Shipton, W. A.
Pue, A.
Fletcher, M. T.
Warner, J. M.
Title Haemolytic fungi isolated from sago starch in Papua New Guinea
Journal name MYCOPATHOLOGIA   Check publisher's open access policy
ISSN 0301-486X
Publication date 2010-02
Year available 2009
Sub-type Article (original research)
DOI 10.1007/s11046-009-9235-3
Volume 169
Issue 2
Start page 107
End page 115
Total pages 9
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Collection year 2010
Language eng
Subject C1
030104 Immunological and Bioassay Methods
111504 Pharmaceutical Sciences
Abstract Sago haemolytic disease (SHD) is a rare but often fatal illness linked to consumption of stale sago starch in Papua New Guinea. Although the aetiology of SHD remains unknown, mycotoxins are suspected. This study investigated whether fungi isolated from Papua New Guinean sago starch were haemolytic. Filamentous fungi and yeasts from sago starch were grown on sheep blood agar and some on human blood agar. Clear haemolytic activity was demonstrated by 55% of filamentous fungal isolates, but not by yeasts. A semi-quantitative bioassay was developed involving incubation of human erythrocytes with fungal extracts. Extracts of cultures of Penicillium, Aspergillus and Fusarium all caused rapid haemolysis in the bioassay. Partial fractionation of extracts suggested that both polar and non-polar haemolytic components had haemolytic activity in vitro. Further work is warranted to identify these metabolites and determine if they play a role in SHD.
Keyword Sago starch
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Published online September 03, 2009

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Created: Sun, 07 Feb 2010, 00:01:42 EST