Profiling humoral immune responses to P. falciparum infection with protein microarrays

Doolan, Denise L., Mu, Yunxiang, Una, Berkay, Sundaresh, Suman, Hirst, Siddiqua, Valdez, Conrad and et al. (2008) Profiling humoral immune responses to P. falciparum infection with protein microarrays. Proteomics, 8 22: 4680-4694. doi:10.1002/pmic.200800194

Author Doolan, Denise L.
Mu, Yunxiang
Una, Berkay
Sundaresh, Suman
Hirst, Siddiqua
Valdez, Conrad
et al.
Title Profiling humoral immune responses to P. falciparum infection with protein microarrays
Journal name Proteomics   Check publisher's open access policy
ISSN 1615-9853
Publication date 2008-11
Sub-type Article (original research)
DOI 10.1002/pmic.200800194
Volume 8
Issue 22
Start page 4680
End page 4694
Total pages 15
Place of publication Weinheim, Germany
Publisher Wiley-VCH
Language eng
Subject 110702 Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies)
Formatted abstract
A complete description of the serological response following exposure of humans to complex pathogens is lacking and approaches suitable for accomplishing this are limited. Here we report,using malaria as a model, a method which elucidates the profile of antibodies that develop after natural or experimental infection or after vaccination with attenuated organisms, and which identifies immunoreactive antigens of interest for vaccine development or other applications. Expression vectors encoding 250 Plasmodium falciparum (Pf) proteins were generated by PCR/recombination cloning; the proteins were individually expressed with .90% efficiency in Escherichia coli cell-free in vitro transcription and translation reactions, and printed directly without purification onto microarray slides. The protein microarrays were probed with human sera from one of four groups which differed in immune status: sterile immunity or no immunity against experimental challenge following vaccination with radiation-attenuated Pf sporozoites, partial immunity acquired by natural exposure, and no previous exposure to Pf. Overall, 72 highly reactive Pf antigens were identified. Proteomic features associated with immunoreactivity were identified. Importantly, antibody profiles were distinct for each donor group. Information obtained from such analyses will facilitate identifying antigens for vaccine development, dissecting the molecular basis of immunity, monitoring the outcome of whole-organism vaccine trials, and identifying immune correlates of protection.
Keyword Antigen identification
Plasmodium falciparum
Protein chip
Proteome microarray
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
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Created: Tue, 02 Feb 2010, 12:41:30 EST by Christine Ouslinis on behalf of Faculty Of Health Sciences