Rapid extraction combined with LC-tandem mass spectrometry (CREM-LC/MS/MS) for the determination of ciguatoxins fish flesh

Lewis, Richard J., Yang, Aijun and Jones, Alun (2009) Rapid extraction combined with LC-tandem mass spectrometry (CREM-LC/MS/MS) for the determination of ciguatoxins fish flesh. Toxicon, 54 1: 62-66. doi:10.1016/j.toxicon.2009.03.013


Author Lewis, Richard J.
Yang, Aijun
Jones, Alun
Title Rapid extraction combined with LC-tandem mass spectrometry (CREM-LC/MS/MS) for the determination of ciguatoxins fish flesh
Journal name Toxicon   Check publisher's open access policy
ISSN 0041-0101
Publication date 2009-03
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.toxicon.2009.03.013
Volume 54
Issue 1
Start page 62
End page 66
Total pages 4
Editor Alan L. Harvey
Place of publication Oxford , U.K
Publisher Elsevier
Collection year 2010
Language eng
Subject 970106 Expanding Knowledge in the Biological Sciences
111705 Environmental and Occupational Health and Safety
111715 Pacific Peoples Health
Abstract Ciguatera is a significant food borne disease caused by potent polyether toxins known as ciguatoxins, which accumulate in the flesh of ciguateric fish at risk levels above 0.1 ppb. The management of ciguatera has been hindered by the lack of analytical methods to detect and quantify clinically relevant levels of ciguatoxin in easily prepared crude extracts of fish. Here we report a ciguatoxin rapid extraction method (CREM) that allows the rapid preparation of fish flesh extracts for the detection and quantification of ciguatoxin by gradient reversed-phase liquid chromatography-tandem mass spectrometry (LC/MS/MS). CREM-LC/MS/MS delivers a linear response to P-CTX-1 spiked into fish prior to extraction. A similar response was obtained for P-CTX-1 spiked after extraction, indicating >95% extraction efficiency was achieved overall and 85% at the limit of quantification (0.1 ppb). Using this approach, levels ≥0.1 ppb P-CTX-1 could be detected and quantified from an extract of 2 g fish flesh, making it suitable as a confirmatory assay for suspect ciguateric carnivorous fish in the Pacific Ocean. The approach is designed to simplify the extraction and analysis of multiple samples per day.
Keyword Analytical detection
Ciguatera
HPLC
Monitoring
Multiple reactant monitoring
Solid phase extraction
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Referred to by: Erratum to ‘‘Rapid extraction combined with LC-tandem mass spectrometry (CREM-LC/MS/MS) for the determination of ciguatoxins in ciguateric fish flesh’’ [Toxicon 54 (2009) 62–66] Toxicon, Volume 54, Issue 6, November 2009, Page 897, Richard J. Lewis, Aijun Yang, Alun Jones

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 29 Jan 2010, 11:42:59 EST by Susan Allen on behalf of Institute for Molecular Bioscience