Antibody production and immunocytochemical localization of amino acid transporters

Pow, David V., Sullivan, Robert and Scott, Heather (2003). Antibody production and immunocytochemical localization of amino acid transporters. In John M. Walker (Ed.), Membrane Transporters (pp. 213-244) Totowa, N.J., U.S.: Humana Press. doi:10.1385/1-59259-387-9:213

Author Pow, David V.
Sullivan, Robert
Scott, Heather
Title of chapter Antibody production and immunocytochemical localization of amino acid transporters
Title of book Membrane Transporters
Place of Publication Totowa, N.J., U.S.
Publisher Humana Press
Publication Year 2003
Sub-type Other
DOI 10.1385/1-59259-387-9:213
Series Methods in Molecular Biology
ISBN 978-1-58829-104-2
Editor John M. Walker
Volume number 227
Chapter number 12
Start page 213
End page 244
Total pages 32
Total chapters 19
Language eng
Subjects 06 Biological Sciences
0601 Biochemistry and Cell Biology
Abstract/Summary This chapter details the procedural and conceptual issues that underpin the localization of high-affinity amino acid transporters by immunocytochemistry, with particular reference to the mammalian nervous system. Defining a role for transporters in any individual tissue often requires an understanding of the spatial distribution of a specific transport system. Techniques such as in situ hybridization provide clear evidence for the potential expression of a transporter by a specific cell type. In many cases however, properties such as targeting to basal or apical surfaces of, for instance, a transport epithelium or targeting to distinct plasmalemmal or cytoplasmic compartments in response to stimulation are most readily determined by the use of antibodies to detect such transporters, in intact tissues, isolated cells, or subcellular fractions of tissues. Methodologies for creating antibodies for the direct immunocytochemical localization of transporters are presented in this chapter. Issues that influence the applicability of methodologies such as postmortem delay, especially in human tissues, are also addressed.
Keyword Amino acid
Nervous system
Q-Index Code BX
Q-Index Status Provisional Code
Institutional Status Unknown

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Created: Wed, 27 Jan 2010, 11:44:28 EST by Ms May Balasaize on behalf of Queensland Brain Institute