The bioremediator glycerophosphodiesterase employs a non-processive mechanism for hydrolysis

Hadler, Kieran S., Gahan, Lawrence R., Ollis, David L. and Schenk, Gerhard (2009) The bioremediator glycerophosphodiesterase employs a non-processive mechanism for hydrolysis. Journal of Inorganic Biochemistry, 104 2: 211-213. doi:10.1016/j.jinorgbio.2009.10.012

Author Hadler, Kieran S.
Gahan, Lawrence R.
Ollis, David L.
Schenk, Gerhard
Title The bioremediator glycerophosphodiesterase employs a non-processive mechanism for hydrolysis
Journal name Journal of Inorganic Biochemistry   Check publisher's open access policy
ISSN 0162-0134
Publication date 2009-10-23
Sub-type Article (original research)
DOI 10.1016/j.jinorgbio.2009.10.012
Volume 104
Issue 2
Start page 211
End page 213
Total pages 3
Editor Dr. J H Dawson
Place of publication United States
Publisher Elsevier Inc.
Collection year 2010
Language eng
Subject C1
030201 Bioinorganic Chemistry
970103 Expanding Knowledge in the Chemical Sciences
Abstract Glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes is a binuclear metallohydrolase that catalyzes the breakdown of a broad range of phosphate ester substrates, and it is of interest for its potential application in the destruction of organophosphate nerve agents and pesticides. The reaction mechanism of GpdQ has been proposed to involve a nucleophilic attack by a terminally bound hydroxide molecule. The hydroxide species bridging the two metal ions is suggested to activate the nucleophile, thus favoring a sequential rather than a processive mechanism of action. Here, the hydrolysis of the two ester bonds in the substrate bis(para-nitrophenyl) phosphate (bpNPP) is probed using 31P NMR. The kinetic rates measured compare well with those determined spectrophotometrically. Furthermore, the data indicate that the diester bonds are cleaved in two separate (non-processive) reactions, indicating that only a single nucleophile (the terminal hydroxide molecule) is likely to be employed as a nucleophile for GpdQ.
Keyword Binuclear metallohydrolases
P-31 NMR
Sequential mechanism
Processive mechanism
Reaction mechanism
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
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Created: Sun, 24 Jan 2010, 00:02:16 EST