The SMN Interactome Includes Myb-Binding Protein 1a

Fuller, HR, Man, NT, Lam, LT, Thanh, LT, Keough, RA, Asperger, A, Gonda, TJ and Morris, GE (2010) The SMN Interactome Includes Myb-Binding Protein 1a. JOURNAL OF PROTEOME RESEARCH, 9 1: 556-563. doi:10.1021/pr900884g


Author Fuller, HR
Man, NT
Lam, LT
Thanh, LT
Keough, RA
Asperger, A
Gonda, TJ
Morris, GE
Title The SMN Interactome Includes Myb-Binding Protein 1a
Journal name JOURNAL OF PROTEOME RESEARCH   Check publisher's open access policy
ISSN 1535-3893
Publication date 2010-01
Year available 2009
Sub-type Article (original research)
DOI 10.1021/pr900884g
Volume 9
Issue 1
Start page 556
End page 563
Total pages 8
Editor Hancock, W. S.
Place of publication United States
Publisher American Chemical Society
Collection year 2010
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
970111 Expanding Knowledge in the Medical and Health Sciences
0601 Biochemistry and Cell Biology
Abstract Understanding networks of interacting proteins is a major goal in cell biology. The survival of motor neurons protein (SMN) interacts, directly or indirectly, with a large number of other proteins and reduced levels of SMN cause the inherited disorder spinal muscular atrophy (SMA). Some SMN interactions are stable and stoichiometric, such as those with gemins, while others are expected to be transient and substoichiometric, such as the functional interaction of SMN with coilin in Cajal bodies. This study set out to determine whether novel components of the extensive SMN interactome can be identified by a proteomic approach. SMN complexes were immuno-precipitated from HeLa nuclear extracts, using anti-SMN monoclonal antibody attached to magnetic beads, digested with trypsin, separated by capillary-liquid chromatography and analyzed by MALDI TOF/TOF mass spectrometry. One-hundred and one proteins were detected with a p value of <0.05, SMN, gemins and U snRNPs being the dominant "hits". Sixty-nine of these were rejected after MALDI analysis of two control pull-downs using antibodies against unrelated nuclear proteins. The proteins found only in anti-SMN pulldowns were either known SMN partners, and/or contained dimethylated RG domains involved in direct interaction with the SMN tudor domain, or they were known binding partners of such direct SMN interactors. Myb-binding protein 1 a, identified as a novel candidate, is a mainly nucleolar protein of unknown function but it partially colocalized with SMN in Cajal bodies in HeLa cell nucleoplasm and, like SMN, was reduced in cells from an SMA patient.
Keyword SMN
Myb-binding protein 1a
Cajal body
nucleolus
MALDI TOF/TOF
mass spectrometry
immunoprecipitation
interaction
SMA
SPINAL MUSCULAR-ATROPHY
MOTOR-NEURON COMPLEX
DETERMINING GENE-PRODUCT
RNA-POLYMERASE-II
MESSENGER-RNA
COILED BODIES
NUCLEAR GEMS
CAJAL BODIES
HELICASE-A
IN-VITRO
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
UQ Diamantina Institute Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 16 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 24 Jan 2010, 00:01:54 EST