PnCcp, a Phytophthora nicotianae protein containing a single complement control protein module, is sorted into large peripheral vesicles in zoospores

Škalamera, D. and Hardham, A. R. (2006) PnCcp, a Phytophthora nicotianae protein containing a single complement control protein module, is sorted into large peripheral vesicles in zoospores. Australasian Plant Pathology, 35 6: 593-603. doi:10.1071/AP06063


Author Škalamera, D.
Hardham, A. R.
Title PnCcp, a Phytophthora nicotianae protein containing a single complement control protein module, is sorted into large peripheral vesicles in zoospores
Formatted title
PnCcp, a Phytophthora nicotianae protein containing a single complement control protein module, is sorted into large peripheral vesicles in zoospores
Journal name Australasian Plant Pathology   Check publisher's open access policy
ISSN 0815-3191
1448-6032
Publication date 2006
Sub-type Article (original research)
DOI 10.1071/AP06063
Volume 35
Issue 6
Start page 593
End page 603
Total pages 11
Place of publication Collingwood, Vic., Australia
Publisher CSIRO
Language eng
Subject 0607 Plant Biology
Abstract Motile zoospores are instrumental in establishing infection in many species of Phytophthora and other pathogenic oomycetes.We have identified Phytophthora nicotianae complement control protein (PnCcp), a protein that occurs in the large peripheral vesicles in Phytophthora nicotianae zoospores. Phytophthora nicotianae PnCcp contains a single complement control protein module and has homologues in P. infestans, P. sojae and P. ramorum. Sequence analysis revealed that PnCcp is a single copy, intron-less gene. Unlike the promoters of other Phytophthora genes that have been described, the PnCcp promoter contains a putative non-canonical TATA box, a CT boxcontaining initiator element and a downstream promoter element. PnCcp was expressed at low levels in mycelia and germinated cysts butwas significantly upregulated in sporulating hyphae and zoospores. Immunolocalisation studies in zoospores and sporangia showed that PnCcp colocalised with LPV, a high molecular weight glycoprotein that is resident in the large peripheral vesicles. However, in mycelia arising from cysts that had germinated up to 3 days earlier, the two proteins did not colocalise, suggesting that the large peripheral vesicles may contain components that are processed separately during the asexual life cycle. © Australasian Plant Pathology Society 2006
Keyword Secretory vesicles
PnCcp
Phytophthora nicotianae complement control protein
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
UQ Diamantina Institute Publications
 
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Created: Thu, 21 Jan 2010, 15:38:23 EST by Natalie Holt on behalf of UQ Diamantina Institute