Association of vitamin d receptor genotypes with calcium excretion in nephrolithiatic subjects in northern India

Relan, Vandana, Khullar, Madhu, Singh, S. K. and Sharma, S. K. (2004) Association of vitamin d receptor genotypes with calcium excretion in nephrolithiatic subjects in northern India. Urological Research, 32 3: 236-240. doi:10.1007/s00240-004-0414-x


Author Relan, Vandana
Khullar, Madhu
Singh, S. K.
Sharma, S. K.
Title Association of vitamin d receptor genotypes with calcium excretion in nephrolithiatic subjects in northern India
Journal name Urological Research   Check publisher's open access policy
ISSN 0300-5623
1434-0879
Publication date 2004-06
Sub-type Article (original research)
DOI 10.1007/s00240-004-0414-x
Volume 32
Issue 3
Start page 236
End page 240
Total pages 5
Place of publication Berlin, Germany
Publisher Springer-Verlag
Language eng
Subject 1103 Clinical Sciences
Formatted abstract
Our objective was to investigate the association between the vitamin D receptor (VDR) allelic variants (Bsm I and Fok I) and nephrolithiasis in northern India. A total of 150 nephrolithiatic patients and 100 age and sex matched controls were enrolled for study. A 10 ml blood sample was obtained for biochemical analysis and DNA isolation. In addition, 24 h urine samples were obtained from each patient for the estimation of calcium and creatinine. PCR was performed for the Bsm I and Fok I VDR variants. The association between Bsm I and Fok I VDR polymorphism and nephrolithiasis was investigated after digestion with restriction enzymes (3 U). The product was analysed on 3% agarose gel for Bsm I and 15% polyacrylamide gel for Fok I allelic variants. We did not observe any significant differences in the prevalence of either the Bsm I or Fok I VDR genotypes between stone formers and controls. The B allele was found to be more prevalent in hypercalciuric patients compared to controls and nephrolithiatic subjects. The subjects with the bb genotype exhibited a higher calcium excretion than the BB genotype. Patients with the F allele were also found to excrete higher urinary calcium. VDR genotypes may be associated with increased calcium excretion in hypercalciuric nephrolithiatic subjects.
© Springer-Verlag 2004
Keyword Vitamin D receptor
Genotypes
Bsm I
Fok I
Calcium excretion
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Thu, 21 Jan 2010, 09:52:38 EST by Jon Swabey on behalf of Faculty Of Health Sciences