Influence of adult Schwann cells and olfactory ensheathing glia on axontarget cell interactions in the CNS a comparative analysis using a retinotectal cograft model

Vukovic, Jana, Plant, Giles W., Ruitenberg, Marc J. and Harvey, Alan R. (2007) Influence of adult Schwann cells and olfactory ensheathing glia on axontarget cell interactions in the CNS a comparative analysis using a retinotectal cograft model. Neuron Glia Biology, 3 2: 105-117. doi:10.1017/S1740925X07000671


Author Vukovic, Jana
Plant, Giles W.
Ruitenberg, Marc J.
Harvey, Alan R.
Title Influence of adult Schwann cells and olfactory ensheathing glia on axontarget cell interactions in the CNS a comparative analysis using a retinotectal cograft model
Journal name Neuron Glia Biology   Check publisher's open access policy
ISSN 1741-0533
1740-925X
Publication date 2007-05
Sub-type Article (original research)
DOI 10.1017/S1740925X07000671
Volume 3
Issue 2
Start page 105
End page 117
Total pages 13
Place of publication Cambridge , U.K.
Publisher Cambridge University Press
Language eng
Subject 0601 Biochemistry and Cell Biology
Abstract We used an in vivo transplant approach to examine how adult Schwann cells and olfactory ensheathing glia OEG influence the specificity of axontarget cell interactions when they are introduced into the CNS. Populations of either Schwann cells or OEG were mixed with dissociated fetal tectal cells presumptive superior colliculus and, after reaggregation, pieces were grafted onto newborn rat superior colliculus. Both glial types were prelabeled with lentiviral vectors encoding green fluorescent protein. Grafts rapidly established fiber connections with the host and retinal projections into cografts were assessed 656 days posttransplantation by injecting cholera toxinB into host eyes. In control rats that received pure dissociatedreaggregated tectal grafts, retinal ganglion cell RGC axons selectively innervated defined target areas, corresponding to the retinorecipient layer in normal superior colliculus. The pattern of RGC axon ingrowth into OEG containing cografts was similar to that in control grafts. However, in Schwann cell cografts there was reduced host retinal input into presumptive target areas and many RGC axons were scattered throughout the neuropil. Given that OEG in cografts had minimal impact on axontarget cell recognition, OEG might be an appropriate cell type for direct transplantation into injured neuropil when attempting to stimulate specific pathway reconstruction.
Keyword Glia
Regeneration
Axonal guidance
Transplantation
Retinal ganglion cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Queensland Brain Institute Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 15 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 20 Jan 2010, 16:34:07 EST by Gerald Martin on behalf of Faculty of Science