TcpA, an FtsK/SpoIIIE Homolog, Is Essential for Transfer of the Conjugative Plasmid pCW3 in Clostridium perfringens

Parsons, Jennifer A., Bannam, Trudi L., Devenish, Rodney J. and Rood, Julian I. (2007) TcpA, an FtsK/SpoIIIE Homolog, Is Essential for Transfer of the Conjugative Plasmid pCW3 in Clostridium perfringens. Journal of Bacteriology, 189 21: 7782-7790. doi:10.1128/JB.00783-07

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Author Parsons, Jennifer A.
Bannam, Trudi L.
Devenish, Rodney J.
Rood, Julian I.
Title TcpA, an FtsK/SpoIIIE Homolog, Is Essential for Transfer of the Conjugative Plasmid pCW3 in Clostridium perfringens
Journal name Journal of Bacteriology   Check publisher's open access policy
ISSN 1098-5530
0021-9193
Publication date 2007-11
Sub-type Article (original research)
DOI 10.1128/JB.00783-07
Open Access Status File (Publisher version)
Volume 189
Issue 21
Start page 7782
End page 7790
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society for Microbiology.
Language eng
Abstract The conjugative tetracycline resistance plasmid pCW3 is the paradigm conjugative plasmid in the anaerobic gram-positive pathogen Clostridium perfringens. Two closely related FtsK/SpoIIIE homologs, TcpA and TcpB, are encoded on pCW3, which is significant since FtsK domains are found in coupling proteins of gram-negative conjugation systems. To develop an understanding of the mechanism of conjugative transfer in C. perfringens, we determined the role of these proteins in the conjugation process. Mutation and complementation analysis was used to show that the tcpA gene was essential for the conjugative transfer of pCW3 and that the tcpB gene was not required for transfer. Furthermore, complementation of a pCW3{Delta}tcpA mutant with divergent tcpA homologs provided experimental evidence that all of the known conjugative plasmids from C. perfringens use a similar transfer mechanism. Functional genetic analysis of the TcpA protein established the essential role in conjugative transfer of its Walker A and Walker B ATP-binding motifs and its FtsK-like RAAG motif. It is postulated that TcpA is the essential DNA translocase or coupling protein encoded by pCW3 and as such represents a key component of the unique conjugation process in C. perfringens.
Keyword Anaerobic gram-positive pathogen
Clostridium Perfringens
FtsK domains
C. perfringens
Mutation
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Tue, 19 Jan 2010, 09:38:48 EST by Rosalind Blair on behalf of Aust Institute for Bioengineering & Nanotechnology