Identification of anthocyanin in Bayberry (Myrica rubra Sieb. et Zucc.) by HPLC-DAD-ESIMS and GC

Fang, Zhong-Xiang, Zhang, Min, Wang, Lin-Xiang and Sun, Jing-Cai (2006) Identification of anthocyanin in Bayberry (Myrica rubra Sieb. et Zucc.) by HPLC-DAD-ESIMS and GC. Journal of Food and Drug Analysis, 14 4: 368-372.

Author Fang, Zhong-Xiang
Zhang, Min
Wang, Lin-Xiang
Sun, Jing-Cai
Title Identification of anthocyanin in Bayberry (Myrica rubra Sieb. et Zucc.) by HPLC-DAD-ESIMS and GC
Formatted title
 Identification of anthocyanin in Bayberry (Myrica rubra Sieb. et Zucc.) by HPLC-DAD-ESIMS and GC
Journal name Journal of Food and Drug Analysis   Check publisher's open access policy
ISSN 1021-9498
Publication date 2006
Year available 2006
Sub-type Article (original research)
Open Access Status Not Open Access
Volume 14
Issue 4
Start page 368
End page 372
Total pages 5
Place of publication Taiwan
Publisher Bureau of Food and Drug Analysis
Language eng
Subject 0703 Crop and Pasture Production
0799 Other Agricultural and Veterinary Sciences
Abstract Bayberry (Myrica rubra Sieb. et Zucc.) anthocyanins were extracted with 0.15% HCl in methanol, removed from other polyphenolsby partition with petroleum ether and ethyl acetate, and then purified in a C-18 solid phase cartridge. Pigments were analyzed by high-performance liquid chromatography (HPLC), photodiode array detection (DAD), electronic spray ion mass spectrometry(ESIMS) and gas chromatography (GC) without the standard. The major anthocyanin, which contained 97% of the total peak area, was identified as cyanidin 3-glucoside. This pigment may be taken as a marker for authenticity control of bayberry products and has potential as a reference compound of cyanidin 3-glucoside in anthocyanin analysis. When standard chemicals are not available, HPLC-DAD-ESIMS and GC are useful in anthocyanin identification if the anthocyanin profile is not overly complex in the studied materials.
Keyword Bayberry
Anthocyanin
HPLC-DAD-ESIMS
GC
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Agriculture and Food Sciences
 
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Created: Mon, 18 Jan 2010, 09:46:30 EST by Macushla Boyle on behalf of Faculty Of Nat Resources, Agric & Veterinary Sc