Cryptic expression of functional glutamate transporters in the developing rodent brain

Williams, Susan M., Macnab, Lauren T. and Pow, David V. (2006) Cryptic expression of functional glutamate transporters in the developing rodent brain. Neuron Glia Biology, 2 3: 199-215. doi:10.1017/S1740925X06000263

Author Williams, Susan M.
Macnab, Lauren T.
Pow, David V.
Title Cryptic expression of functional glutamate transporters in the developing rodent brain
Journal name Neuron Glia Biology   Check publisher's open access policy
ISSN 1740-925X
Publication date 2006-08
Sub-type Article (original research)
DOI 10.1017/S1740925X06000263
Volume 2
Issue 3
Start page 199
End page 215
Total pages 7
Place of publication Cambridge, United Kingdom
Publisher Cambridge University Press
Language eng
Subject 1109 Neurosciences
Abstract The co-ordinate functioning of neurons and glia is required for glutamate-mediated neurotransmission. In this study, we show by immunocytochemical detection of D-aspartate uptake, that functional glutamate transporters are present in the developing CNS of fetal and neonatal rats, including forebrain, midbrain and hindbrain, at least as early as embryonic day 12 (E12). Use of the transport inhibitor dihydrokainic acid revealed a significant role for GLT-1 in the uptake process. Immunolabelling for the glutamate transporters GLAST, GLT-1α and GLT-1v showed that each of these proteins are expressed early in development and appear to be restricted to glial-like cells throughout the development period examined (except in the retina, where neuronal elements were also labelled). Our capacity to detect very early expression of the variant forms of GLT-1 contrasts with other studies, a feature that we attribute to the use of antigen-recovery techniques that unmask protein epitopes that are otherwise undetectable. These studies illustrate the widespread presence of functional glutamate transporters in the developing CNS, in many cases before the onset of periods of synaptogenesis and indicate that regulation of extracellular glutamate by multiple excitatory amino acid transporters might be crucial in early CNS development. ©2007 Cambridge University Press
Keyword D-aspartate
Glutamate transport
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
UQ Centre for Clinical Research Publications
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Created: Mon, 18 Jan 2010, 09:28:24 EST by Jon Swabey on behalf of Faculty Of Health Sciences