Neo-vascularization and bone formation mediated by fetal mesenchymal stem cell tissue-engineered bone grafts in critical-size femoral defects

Zhang, Zhi-Yong, Teoh, Swee-Hin, Chong, Mark S. K., Lee, Eddy S. M., Tan, Lay-Geok, Mattar, Citra N., Fisk, Nicholas M., Choolani, Magesh and Chan, Jerry (2010) Neo-vascularization and bone formation mediated by fetal mesenchymal stem cell tissue-engineered bone grafts in critical-size femoral defects. Biomaterials, 31 4: 608-620. doi:10.1016/j.biomaterials.2009.09.078


Author Zhang, Zhi-Yong
Teoh, Swee-Hin
Chong, Mark S. K.
Lee, Eddy S. M.
Tan, Lay-Geok
Mattar, Citra N.
Fisk, Nicholas M.
Choolani, Magesh
Chan, Jerry
Title Neo-vascularization and bone formation mediated by fetal mesenchymal stem cell tissue-engineered bone grafts in critical-size femoral defects
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
1878-5905
Publication date 2010-02
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2009.09.078
Volume 31
Issue 4
Start page 608
End page 620
Total pages 13
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2011
Language eng
Formatted abstract
Tissue-engineered bone grafts (TEBG) require highly osteogenic cell sources for use in fracture repair
applications. Compared to other sources of mesenchymal stem cells (MSC), human fetal MSC (hfMSC)
have recently been shown to be more proliferative and osteogenic. We studied the functional performance
of hfMSC-mediated TEBG in 7 mm rat femoral critical-sized bone defects (CSD). Dynamicallycultured
and osteogenically-primed hfMSC seeded onto macroporous poly-ε-caprolactone tri-calcium
phosphate scaffolds were transplanted into CSDs. After 12 weeks, hfMSC-mediated TEBG induced 2.1
more new bone formation (43.3 ±10.5 vs. 21.0 ± 7.4 mm3, p < 0.05), with greater compact and woven
bone, and a 9.8 increase in stiffness (3.9 ± 1.7 vs. 0.4 ± 0.3 mNm/degree, p < 0.05) compared to acellular
scaffolds, such that only animals transplanted with TEBG underwent full fracture repair of the CSD.
Although hfMSC survived for <4 weeks, by 4 weeks they were associated with a 3.9 larger vasculature
network in the defect area (35.2 ± 11.1 vs. 6.5 ± 3.6 mm3 p < 0.05), suggesting an important role for
hfMSC in the promotion of neo-vasculogenesis.We speculate that hfMSC-mediated healing of the CSD by
stimulating neo-vascularization through as yet undetermined mechanisms. This proof-of-principle study
demonstrates the utility of primitive MSC for bone regeneration, and may be of relevance to vascularization
in other areas of regenerative medicine.
2009 Elsevier Ltd. All rights reserved.
Keyword Fetal mesenchymal stem cells
Bone tissue engineering
Poly-caprolactone
Rat femur
Critical size defect
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 15 October 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2011 Collection
 
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Created: Sun, 17 Jan 2010, 00:07:38 EST