Bridging the synaptic gap: Neuroligins and neurexin I in Apis mellifera

Biswas, Sunita, Russell, Robyn J., Jackson, Colin J., Vidovic, Maria, Ganeshina, Olga, Oakeshott, John G. and Claudianos, Charles (2008) Bridging the synaptic gap: Neuroligins and neurexin I in Apis mellifera. PLoS ONE, 3 10: e3542-1-e3542-19. doi:10.1371/journal.pone.0003542

Author Biswas, Sunita
Russell, Robyn J.
Jackson, Colin J.
Vidovic, Maria
Ganeshina, Olga
Oakeshott, John G.
Claudianos, Charles
Title Bridging the synaptic gap: Neuroligins and neurexin I in Apis mellifera
Formatted title
Bridging the synaptic gap: Neuroligins and neurexin I in Apis mellifera
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2008-10
Sub-type Article (original research)
DOI 10.1371/journal.pone.0003542
Open Access Status DOI
Volume 3
Issue 10
Start page e3542-1
End page e3542-19
Total pages 19
Editor Seth G. N. Grant
Place of publication San Francisco, CA, U.S.A.
Publisher Public Library of Science
Language eng
Subject 1109 Neurosciences
Formatted abstract
Vertebrate studies show neuroligins and neurexins are binding partners in a trans-synaptic cell adhesion complex, implicated in human autism and mental retardation disorders. Here we report a genetic analysis of homologous proteins in the honey bee. As in humans, the honeybee has five large (31–246 kb, up to 12 exons each) neuroligin genes, three of which are tightly clustered. RNA analysis of the neuroligin-3 gene reveals five alternatively spliced transcripts, generated through alternative use of exons encoding the cholinesterase-like domain. Whereas vertebrates have three neurexins the bee has just one gene named neurexin I (400 kb, 28 exons). However alternative isoforms of bee neurexin I are generated by differential use of 12 splice sites, mostly located in regions encoding LNS subdomains. Some of the splice variants of bee neurexin I resemble the vertebrate α- and β-neurexins, albeit in vertebrates these forms are generated by alternative promoters. Novel splicing variations in the 3′ region generate transcripts encoding alternative trans-membrane and PDZ domains. Another 3′ splicing variation predicts soluble neurexin I isoforms. Neurexin I and neuroligin expression was found in brain tissue, with expression present throughout development, and in most cases significantly up-regulated in adults. Transcripts of neurexin I and one neuroligin tested were abundant in mushroom bodies, a higher order processing centre in the bee brain. We show neuroligins and neurexins comprise a highly conserved molecular system with likely similar functional roles in insects as vertebrates, and with scope in the honeybee to generate substantial functional diversity through alternative splicing. Our study provides important prerequisite data for using the bee as a model for vertebrate synaptic development.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Article no. e3542, pp.1-19

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
ERA 2012 Admin Only
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 35 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 17 Jan 2010, 00:00:36 EST