Measurements of aneurysm morphology determined by 3-d micro-ultrasound imaging as potential quantitative biomarkers in a mouse aneurysm model

Goldberg, Alyse, Pakkiri, Pria, Dai, Erbin, Lucas, Alexandra and Fenster, Aaron (2007) Measurements of aneurysm morphology determined by 3-d micro-ultrasound imaging as potential quantitative biomarkers in a mouse aneurysm model. Ultrasound in Medicine & Biology, 33 10: 1552-1560. doi:10.1016/j.ultrasmedbio.2007.04.003


Author Goldberg, Alyse
Pakkiri, Pria
Dai, Erbin
Lucas, Alexandra
Fenster, Aaron
Title Measurements of aneurysm morphology determined by 3-d micro-ultrasound imaging as potential quantitative biomarkers in a mouse aneurysm model
Journal name Ultrasound in Medicine & Biology   Check publisher's open access policy
ISSN 0301-5629
1879-291X
Publication date 2007-10
Sub-type Article (original research)
DOI 10.1016/j.ultrasmedbio.2007.04.003
Volume 33
Issue 10
Start page 1552
End page 1560
Total pages 9
Place of publication London, U.K.
Publisher Elsevier
Language eng
Subject 1109 Neurosciences
Formatted abstract
Aneurysms remain a significant medical problem and our current understanding of aneurysm formation and developmental stages remains incomplete. Noninvasive 3-D micro-ultrasound (3-D micro-US) imaging technologies designed for noninvasive evaluation of small laboratory animals diminish risks associated with invasive examination and provide in-situ (live) analysis of vascular morphological changes, which enables quantitative measurements of live biological specimens. We demonstrate here that aneurysm morphology can be quantified using 3-D micro-US, and we validate this methodology through comparison of geometric measures with those obtained from 3-D serial histologic records in a mouse model of accelerated aneurysm formation. Aneurysms were induced in Balb/C mice after C57Bl/6 mouse aortic transplant with injections of a pro-inflammatory viral serpin with a mutated reactive site. Aortic transplant segments were imaged 28 days after transplant using 3-D micro-US. Upon sacrifice, the aortas were excised and histology sections (5-μm thick) were digitized, co-registered using mutual information and stacked to form 3-D images. Surfaces of the mouse aorta and aneurysm were manually segmented from the 3-D micro-US and histology images. Comparisons with 3-D histology images demonstrated that 3-D micro-US allowed in-vivo analysis of aneurysm morphology, including total aneurysm area, plaque growth and lumen size. Linear regression of 3-D US-derived aneurysm and plaque volumes vs. 3-D histology-derived volumes resulted in slopes of 1.30 (R2 = 0.96) and 1.20 (R2 = 0.98), respectively, demonstrating that 3-D micro-US measurements can be used to track aneurysm growth in a mouse aortic transplant model. Copyright © 2007 World Federation for Ultrasound in Medicine & Biology Published by Elsevier Inc.
Keyword 3D Ultrasound
Aneurysm
Mouse imaging
Vascular imaging
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 15 Jan 2010, 12:37:41 EST by Tara Johnson on behalf of Faculty Of Health Sciences