Demonstration of the protective effects of fluorescent proteins in baculoviruses exposed to ultraviolet light inactivation

McIntosh, A. H., Grasela, J. J., Lua, L. and Braunagel, S. C. (2004) Demonstration of the protective effects of fluorescent proteins in baculoviruses exposed to ultraviolet light inactivation. Journal of Insect Science, 4 31: 1-9. doi:10.1673/031.004.3101


Author McIntosh, A. H.
Grasela, J. J.
Lua, L.
Braunagel, S. C.
Title Demonstration of the protective effects of fluorescent proteins in baculoviruses exposed to ultraviolet light inactivation
Journal name Journal of Insect Science   Check publisher's open access policy
ISSN 1536-2442
2250-2645
Publication date 2004-10-05
Sub-type Article (original research)
DOI 10.1673/031.004.3101
Open Access Status DOI
Volume 4
Issue 31
Start page 1
End page 9
Total pages 9
Place of publication Cary, NC, United States
Publisher Oxford University Press
Language eng
Subject 1007 Nanotechnology
Formatted abstract
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) recombinants, namely AcRFP produced by fusion of the red fluorescent protein (RFP) gene with the polyhedrin gene, and a recombinant (pAcUW21-23GFP) carrying the green fluorescent protein (GFP) in its viral envelope, were evaluated for their resistance to inactivation by ultraviolet light. AcRFP recombinants produced incomplete polyhedra with low infectivity for Trichoplusia ni larvae, whereas AcuW21-23GFP produced normal polyhedra with high infectivity. Electron microscopy of AcRFP CL14 showed the incorporation of very few viral particles into polyhedrin matrix protein material. The LC50 for AcuW21-23GFP was 0.10 occlusion bodies/mm2, whereas the LC50 values for several AcRFP recombinants ranged from 20 to 329 occlusion bodies/mm2. When both the RFP and GFP recombinants were exposed to ultraviolet light (UV-B 280-320 nm), the results support the conclusion that these fluorescent proteins afford some protection against its damaging effects.
Keyword AcMNPV
Recombinants
Electron microscopy
Bioassays
Insect cell culture
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Thu, 14 Jan 2010, 13:51:29 EST by Christine Ouslinis on behalf of Aust Institute for Bioengineering & Nanotechnology