A new method for isolating large quantities of Arabidopsis trichomes for transcriptome, cell wall and other types of analyses

Marks, M. David, Betancur, Lissete, Gilding, Edward, Chen, Fang, Bauer, Stefan, Wenger, Jonathan P., Dixon, Richard A. and Haigler, Candace H. (2008) A new method for isolating large quantities of Arabidopsis trichomes for transcriptome, cell wall and other types of analyses. The Plant Journal, 56 3: 483-492.


Author Marks, M. David
Betancur, Lissete
Gilding, Edward
Chen, Fang
Bauer, Stefan
Wenger, Jonathan P.
Dixon, Richard A.
Haigler, Candace H.
Title A new method for isolating large quantities of Arabidopsis trichomes for transcriptome, cell wall and other types of analyses
Journal name The Plant Journal   Check publisher's open access policy
ISSN 0960-7412
1365-313X
Publication date 2008-11
Sub-type Article (original research)
DOI 10.1111/j.1365-313X.2008.03611.x
Volume 56
Issue 3
Start page 483
End page 492
Total pages 10
Place of publication Oxford, U.K.
Publisher BIOS Publishers : Blackwell Scientific in association with the Society for Experimental Biology
Language eng
Subject 0607 Plant Biology
Abstract A new procedure has been developed for the isolation of wild-type and mutant Arabidopsis trichomes. The isolated trichomes maintained enzymatic activity and were used for DNA, protein, and RNA isolation. The RNA was used to generate probes suitable for Affymetrix analysis. The validity of the Affymetrix results was confirmed by quantitative PCR analysis on a subset of genes that are preferentially expressed in trichomes or leaves. Sufficient quantities of trichomes were isolated to probe the biochemical nature of trichome cell walls. These analyses provide evidence for the presence of lignin in Arabidopsis trichome cell walls. The monosaccharide analysis and positive staining with ruthenium red indicates that the walls also contain a large portion of pectin. The 2.23-fold ratio of pectin-related sugars compared with potential cellulosic glucose suggests that the polysaccharides of the trichome cell walls are more like those of typical primary walls even though the wall becomes quite thick. Overall, these analyses open the door to using the Arabidopsis trichome cell wall as an excellent model to probe various questions concerning plant cell wall biosynthesis. © 2009 Blackwell Publishing Ltd and the Society for Experimental Biology.
Keyword Trichome isolation
Trichome cell wall
Trichome gene expression
Lignin
Pectin
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Agriculture and Food Sciences
 
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