Signature-tagged mutagenesis of Pasteurella multocida identifies mutants displaying differential virulence characteristics in mice and chickens

Harper, Marina, Boyce, John D., Wilkie, Ian W. and Adler, Ben (2003) Signature-tagged mutagenesis of Pasteurella multocida identifies mutants displaying differential virulence characteristics in mice and chickens. Infection and Immunity, 71 9: 5440-5446. doi:10.1128/IAI.71.9.5440-5446.2003

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Author Harper, Marina
Boyce, John D.
Wilkie, Ian W.
Adler, Ben
Title Signature-tagged mutagenesis of Pasteurella multocida identifies mutants displaying differential virulence characteristics in mice and chickens
Formatted title
Signature-tagged mutagenesis of Pasteurella multocida identifies mutants displaying differential virulence characteristics in mice and chickens
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 1098-5522
1070-6313
Publication date 2003-09
Sub-type Article (original research)
DOI 10.1128/IAI.71.9.5440-5446.2003
Open Access Status File (Publisher version)
Volume 71
Issue 9
Start page 5440
End page 5446
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
Pasteurella multocida is the causative agent of fowl cholera in birds. Signature-tagged mutagenesis (STM) was used to identify potential virulence factors in a mouse septicemia disease model and a chicken fowl cholera model. A library of P. multocida mutants was constructed with a modified Tn916 and screened for attenuation in both animal models. Mutants identified by the STM screening were confirmed as attenuated by competitive growth assays in both chickens and mice. Of the 15 mutants identified in the chicken model, only 5 were also attenuated in mice, showing for the first time the presence of host-specific virulence factors and indicating the importance of screening for attenuation in the natural host. Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Keyword Outer-membrane protein
Chain fatty-acids
Escherichia-coli
Haemophilus-influenzae
Transposon mutagenesis
Nonspecific adherence
Genes
Purification
Expression
Capsule
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 42 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 12 Jan 2010, 11:55:13 EST by Jon Swabey on behalf of Faculty Of Nat Resources, Agric & Veterinary Sc