A cluster of ring stage-specific genes linked to a locus implicated in cytoadherence in Plasmodium falciparum codes for PEXEL-negative and PEXEL-positive proteins exported into the host cell

Spielmann, Tobias, Hawthorne, Paula L., Dixon, Matthew W. A., Hannemann, Mandy, Klotz, Kathleen, Kemp, David J., Klonis, Nectarios, Tilley, Leann, Trenholme, Katharine R. and Gardiner, Donald L. (2006) A cluster of ring stage-specific genes linked to a locus implicated in cytoadherence in Plasmodium falciparum codes for PEXEL-negative and PEXEL-positive proteins exported into the host cell. Molecular Biology of the Cell, 17 8: 3613-3624. doi:10.1091/mbc.E06-04-0291

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Author Spielmann, Tobias
Hawthorne, Paula L.
Dixon, Matthew W. A.
Hannemann, Mandy
Klotz, Kathleen
Kemp, David J.
Klonis, Nectarios
Tilley, Leann
Trenholme, Katharine R.
Gardiner, Donald L.
Title A cluster of ring stage-specific genes linked to a locus implicated in cytoadherence in Plasmodium falciparum codes for PEXEL-negative and PEXEL-positive proteins exported into the host cell
Journal name Molecular Biology of the Cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2006-08
Sub-type Article (original research)
DOI 10.1091/mbc.E06-04-0291
Open Access Status File (Publisher version)
Volume 17
Issue 8
Start page 3613
End page 3624
Total pages 12
Place of publication Bethesda, U.S.
Publisher American Society for Cell Biology
Language eng
Subject 0601 Biochemistry and Cell Biology
Formatted abstract
Blood stages of Plasmodium falciparum export proteins into their erythrocyte host, thereby inducing extensive host cell modifications that become apparent after the first half of the asexual development cycle (ring stage). This is responsible for a major part of parasite virulence. Export of many parasite proteins depends on a sequence motif termed Plasmodium export element (PEXEL) or vacuolar transport signal (VTS). This motif has allowed the prediction of the Plasmodium exportome. Using published genome sequence, we redetermined the boundaries of a previously studied region linked to P. falciparum virulence, reducing the number of candidate genes in this region to 13. Among these, we identified a cluster of four ring stage-specific genes, one of which is known to encode an exported protein. We demonstrate that all four genes code for proteins exported into the host cell, although only two genes contain an obvious PEXEL/VTS motif. We propose that the systematic analysis of ring stage-specific genes will reveal a cohort of exported proteins not present in the currently predicted exportome. Moreover, this provides further evidence that host cell remodeling is a major task of this developmental stage. Biochemical and photobleaching studies using these proteins reveal new properties of the parasite-induced membrane compartments in the host cell. This has important implications for the biogenesis and connectivity of these structures. Copyright © 2006 by The American Society for Cell Biology.
Keyword Plasmodium falciparum
Cytoadherence
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Equine Genetics Research Centre Publications
 
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Created: Tue, 12 Jan 2010, 10:23:45 EST by Tara Johnson on behalf of Aust Equine Genetics Research Centre