Genetic and optical targeting of neural circuits and behavior - zebrafish in the spotlight

Baier, Herwig and Scott, Ethan K (2009) Genetic and optical targeting of neural circuits and behavior - zebrafish in the spotlight. Current Opinion In Neurobiology, 19 5: 553-560. doi:10.1016/j.conb.2009.08.001

Author Baier, Herwig
Scott, Ethan K
Title Genetic and optical targeting of neural circuits and behavior - zebrafish in the spotlight
Journal name Current Opinion In Neurobiology   Check publisher's open access policy
ISSN 0959-4388
Publication date 2009-10
Sub-type Article (original research)
DOI 10.1016/j.conb.2009.08.001
Volume 19
Issue 5
Start page 553
End page 560
Total pages 8
Editor Marc Tessier-Lavigne
Place of publication United Kingdom
Publisher Elseiver Ltd., Current Opinion Journals
Collection year 2010
Language eng
Subject C1
969902 Marine Oceanic Processes (excl. Climate Related)
050202 Conservation and Biodiversity
Abstract Methods to label neurons and to monitor their activity with genetically encoded fluorescent reporters have been a staple of neuroscience research for several years. The recent introduction of photoswitchable ion channels and pumps, such as channelrhodopsin (ChR2), halorhodopsin (NpHR), and light-gated glutamate receptor (LiGluR), is enabling remote optical manipulation of neuronal activity. The translucent brains of zebrafish offer superior experimental conditions for optogenetic approaches in vivo. Enhancer and gene trapping approaches have generated hundreds of Gal4 driver lines in which the expression of UAS-linked effectors can be targeted to subpopulations of neurons. Local photoactivation of genetically targeted LiGluR, ChR2, or NpHR has uncovered novel functions for specific areas and cell types in zebrafish behavior. Because the manipulation is restricted to times and places where genetics (cell types) and optics (beams of light) intersect, this method affords excellent resolving power for the functional analysis of neural circuitry.
Keyword Fluorescent Protein
Transgenic Zebrafish
Nervous system
Trap approach
Expression light
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biomedical Sciences Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 52 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 56 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 10 Jan 2010, 00:07:48 EST