The continuing contribution of gene marking to cell and gene therapy

Tey, Siok-Keen and Breener, Malcolm K. (2007) The continuing contribution of gene marking to cell and gene therapy. Molecular Therapy, 15 4: 666-676. doi:10.1038/

Author Tey, Siok-Keen
Breener, Malcolm K.
Title The continuing contribution of gene marking to cell and gene therapy
Journal name Molecular Therapy   Check publisher's open access policy
ISSN 1525-0016
Publication date 2007-04
Sub-type Article (original research)
DOI 10.1038/
Volume 15
Issue 4
Start page 666
End page 676
Total pages 11
Place of publication San Diego, CA, United States
Publisher Nature Publishing
Language eng
Subject 11 Medical and Health Sciences
1103 Clinical Sciences
Abstract Gene-marking studies were the first gene-transfer protocols approved for human use. Their intent was not directly therapeutic but rather to track the behavior and fate of cells in vivo, and to use this information to improve treatment protocols. For more than fifteen years, gene-marking studies using retroviral vectors have provided invaluable information about the biology of human hematopoietic cells and T lymphocytes, and have helped guide cell therapies intended to treat malignant disease. Although the safety record of marking studies has been impeccable, the development of leukemia by immunodeficient children treated with retroviral vectors cast a pall over the entire field and essentially brought the era of pure gene-marking studies to an abrupt end. Paradoxically, the impetus these events gave to studying retroviral integration sites in host cell DNA emphasized the additional information that marker studies could provide about the behavior of cells at the clonal level. As confidence has slowly returned, marker studies have reappeared, usually as components of gene therapy protocols in which a marker gene or sequence is incorporated to allow the modified cells to be tracked or imaged in vivo. Hence, gene marking continues to have much to offer in terms of our understanding of the behavior, fate, and safety of gene-modified cells in vivo.
Keyword Gene marking
Gene transfer
Hematopoietic cells
T Lymphocytes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published online 13 February 2007.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 08 Jan 2010, 13:15:38 EST by Ms May Balasaize on behalf of Faculty Of Health Sciences