Simultaneous detection and identification of Candida, Aspergillus, and Cryptococcus species by reverse line blot hybridization

Playford, Geoffrey E., Kong, Fanrong, Sun, Ying, Wang, Hui, Halliday, Catriona and Sorrell, Tania. C. (2006) Simultaneous detection and identification of Candida, Aspergillus, and Cryptococcus species by reverse line blot hybridization. Journal of Clinical Microbiology, 44 3: 876-880. doi:10.1128/JCM.44.3.876-880.2006

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Author Playford, Geoffrey E.
Kong, Fanrong
Sun, Ying
Wang, Hui
Halliday, Catriona
Sorrell, Tania. C.
Title Simultaneous detection and identification of Candida, Aspergillus, and Cryptococcus species by reverse line blot hybridization
Formatted title
Simultaneous detection and identification of Candida, Aspergillus, and Cryptococcus species by reverse line blot hybridization
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1070-633X
1098-660X
Publication date 2006-03
Sub-type Article (original research)
DOI 10.1128/JCM.44.3.876-880.2006
Open Access Status File (Publisher version)
Volume 44
Issue 3
Start page 876
End page 880
Total pages 4
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
We report on a reverse line blot (RLB) assay, utilizing fungal species-specific oligonucleotide probes to hybridize with internal transcribed spacer 2 region sequences amplified using a nested panfungal PCR. Reference and clinical strains of 16 Candida species (116 strains), Cryptococcus neoformans (five strains of Cryptococcus neoformans var. neoformans, five strains of Cryptococcus neoformans var. grubii, and six strains of Cryptococcus gatti), and five Aspergillus species (68 strains) were all correctly identified by the RLB assay. Additional fungal species (16 species and 26 strains) not represented on the assay did not exhibit cross-hybridization with the oligonucleotide probes. In simulated clinical specimens, the sensitivity of the assay for Candida spp. and Aspergillus spp. was 100.5 cells/ml and 102 conidia/ml, respectively. This assay allows sensitive and specific simultaneous detection and identification of a broad range of fungal pathogens.

Keyword Candida
Aspergillus
Cryptococcus
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Fri, 08 Jan 2010, 11:12:36 EST by Natalie Holt on behalf of Faculty Of Health Sciences