Developmental disparity between in vitro-produced and somatic cell nuclear transfer bovine days 14 and 21 embryos: Implications for embryonic loss

Alexopoulos, Natalie I., Maddox-Hyttel, Poul, Tveden-Nyborg, Pernille, D'Cruz, Nancy T., Tecirlioglu, Tayfur R., Cooney, Melissa A., Schauser, Kirsten, Holland, Michael K. and French, Andrew J. (2008) Developmental disparity between in vitro-produced and somatic cell nuclear transfer bovine days 14 and 21 embryos: Implications for embryonic loss. Reproduction, 136 4: 433-445. doi:10.1530/REP-07-0392


Author Alexopoulos, Natalie I.
Maddox-Hyttel, Poul
Tveden-Nyborg, Pernille
D'Cruz, Nancy T.
Tecirlioglu, Tayfur R.
Cooney, Melissa A.
Schauser, Kirsten
Holland, Michael K.
French, Andrew J.
Title Developmental disparity between in vitro-produced and somatic cell nuclear transfer bovine days 14 and 21 embryos: Implications for embryonic loss
Formatted title
Developmental disparity between in vitro-produced and somatic cell nuclear transfer bovine days 14 and 21 embryos: Implications for embryonic loss
Journal name Reproduction   Check publisher's open access policy
ISSN 1470-1626
1741-7899
Publication date 2008-10-01
Sub-type Article (original research)
DOI 10.1530/REP-07-0392
Volume 136
Issue 4
Start page 433
End page 445
Total pages 13
Place of publication Bristol, U.K.
Publisher BioScientifica
Language eng
Subject 0702 Animal Production
Formatted abstract
In ruminants, the greatest period of embryonic loss coincides with the period of elongation when the embryonic disc is formed and gastrulation occurs prior to implantation. The impact of early embryonic mortality is not only a major obstacle to the cattle breeding industry but also impedes the application of new reproductive technologies such as somatic cell nuclear transfer (SCNT). In the present study, days 14 and 21 bovine embryos, generated by either in vitro-production (IVP) or SCNT, performed by either subzonal injection (SUZI) or handmade cloning (HMC), were compared by stereomicroscopy, immunohistochemistry, and transmission electron microscopy to establish in vivo developmental milestones. Following morphological examination, samples were characterized for the presence of epiblast (POU5F1), mesoderm (VIM), and neuroectoderm (TUBB3). On D14, only 25, 15, and 7% of IVP, SUZI, and HMC embryos were recovered from the embryos transferred respectively, and similar low recovery rates were noted on D21, suggesting that most of the embryonic loss had already occurred by D14. A number of D14 IVP, SUZI, and HMC embryos lacked an epiblast, but presented trophectoderm and hypoblast. When the epiblast was present, POU5F1 staining was limited to this compartment in all types of embryos. At the ultrastructural level, SCNT embryos displayed abundant secondary lysosomes and vacuoles, had fewer mitochondria, polyribosomes, tight junctions, desmosomes, and tonofilaments than their IVP counterparts. The staining of VIM and TUBB3 was less distinct in SCNT embryos when compared with IVP embryos, indicating slower or compromised development. In conclusion, SCNT and to some degree, IVP embryos displayed a high rate of embryonic mortality before D14 and surviving embryos displayed reduced quality with respect to ultrastructural features and differentiation markers. Copyright © 2008 Society for Reproduction and Fertility
Keyword Cloning
Bovine
Embryonic development
Embryonic mortality
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Queensland Brain Institute Publications
 
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Created: Thu, 07 Jan 2010, 21:12:50 EST by Jon Swabey on behalf of Queensland Brain Institute