Analysis of the expression of putatively imprinted genes in bovine peri-implantation embryos

Tveden-Nyborg, P. Y., Alexopoulos, N. I., Cooney, M. A., French, A. J., Tecirlioglu, R. T., Holland, M. K., Thomsen, P. D. and D’Cruz, N. T. (2008) Analysis of the expression of putatively imprinted genes in bovine peri-implantation embryos. Theriogenology, 70 7: 1119-1128. doi:10.1016/j.theriogenology.2008.06.033


Author Tveden-Nyborg, P. Y.
Alexopoulos, N. I.
Cooney, M. A.
French, A. J.
Tecirlioglu, R. T.
Holland, M. K.
Thomsen, P. D.
D’Cruz, N. T.
Title Analysis of the expression of putatively imprinted genes in bovine peri-implantation embryos
Journal name Theriogenology   Check publisher's open access policy
ISSN 0093-691X
Publication date 2008-10-15
Sub-type Article (original research)
DOI 10.1016/j.theriogenology.2008.06.033
Volume 70
Issue 7
Start page 1119
End page 1128
Total pages 10
Place of publication New York, U.S.A.
Publisher Elsevier
Language eng
Subject 0707 Veterinary Sciences
Formatted abstract
The application of assisted reproductive technologies (ART) has been shown to induce changes in the methylation of the embryonic genome, leading to aberrant gene expression, including that of imprinted genes. Aberrant methylation and gene expression has been linked to the large offspring syndrome (LOS) in bovine embryos resulting in increased embryonic morbidity and mortality. In the bovine, limited numbers of imprinted genes have been studied and studies have primarily been restricted to pre-implantation stages. This study reports original data on the expression pattern of 8 putatively imprinted genes (Ata3, Dlk1, Gnas, Grb10, Magel2, Mest-1, Ndn and Sgce) in bovine peri-implantation embryos. Two embryonic developmental stages were examined, Day 14 and Day 21. The gene expression pattern of single embryos was recorded for in vivo, in vitro produced (IVP) and parthenogenetic embryos. The IVP embryos allow us to estimate the effect of in vitro procedures and the analysis of parthenogenetic embryos provides provisional information on maternal genomic imprinting. Among the 8 genes investigated, only Mest-1 showed differential expression in Day 21 parthenogenetic embryos compared to in vivo and IVP counterparts, indicating maternal imprinting of this gene. In addition, our expression analysis of single embryos revealed a more heterogeneous gene expression in IVP than in in vivo developed embryos, adding further to the hypothesis of transcriptional dysregulation induced by in vitro procedures, either by in vitro maturation, fertilization or culture. In conclusion, effects of genomic imprinting and of in vitro procedures for embryo production may influence the success of bovine embryo implantation. © 2008 Elsevier Inc. All rights reserved.
Keyword Gene expression
Mest-1
Peri-implantation
Uniparental
Bovine
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Queensland Brain Institute Publications
 
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Created: Thu, 07 Jan 2010, 10:45:45 EST by Jon Swabey on behalf of Queensland Brain Institute