Studies on the synthesis, characterization, binding with DNA and activities of two cis-planaramineplatinum(II) complexes of the form: cis-PtL(NH3)Cl2 where L = 3-hydroxypyridine and 2,3-diaminopyridine

Abdullah, Ahmed, Huq, Fazlul, Chowdhury, Ashraf, Tayyem, Hasan, Beale, Philip and Fisher, Keith (2006) Studies on the synthesis, characterization, binding with DNA and activities of two cis-planaramineplatinum(II) complexes of the form: cis-PtL(NH3)Cl2 where L = 3-hydroxypyridine and 2,3-diaminopyridine. BMC Chemical Biology, 6 3.1-3.9. doi:10.1186/1472-6769-6-3


Author Abdullah, Ahmed
Huq, Fazlul
Chowdhury, Ashraf
Tayyem, Hasan
Beale, Philip
Fisher, Keith
Title Studies on the synthesis, characterization, binding with DNA and activities of two cis-planaramineplatinum(II) complexes of the form: cis-PtL(NH3)Cl2 where L = 3-hydroxypyridine and 2,3-diaminopyridine
Formatted title
Studies on the synthesis, characterization, binding with DNA and activities of two cis-planaramineplatinum(II) complexes of the form: cis-PtL(NH3)Cl2 where L = 3-hydroxypyridine and 2,3-diaminopyridine
Journal name BMC Chemical Biology   Check publisher's open access policy
ISSN 1472-6769
Publication date 2006-03-13
Year available 2006
Sub-type Article (original research)
DOI 10.1186/1472-6769-6-3
Open Access Status DOI
Volume 6
Start page 3.1
End page 3.9
Total pages 9
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Subject 11 Medical and Health Sciences
110105 Medical Biochemistry: Nucleic Acids
Formatted abstract
Background Cis-planaramineplatinum(II) complexes like their trans isomers are often found to be active against cancer cell lines. The present study deals with the synthesis, characterization and determination of activity of new cis-planaramineplatinum(II) complexes.

Results
Two cis-planaramineplatinum(II) complexes: cis-(3-hydroxypyridine)(ammine)dichloroplatinum(II) (code named AH3) and cis-(2,3-diaminopyridine)(ammine)dichloroplatinum(II) (code named AH7) have been prepared and characterised based on elemental analyses, IR, Raman, mass and 1H NMR spectral measurements. The interactions of the compounds with pBR322 plasmid DNA have been investigated and their activity against ovarian cancer cell lines: A2780, A2780cisR and A2780ZD047R have been determined. Like cisplatin, AH3 and AH7 are believed to form mainly monofunctional N7(G) and bifunctional intrastrand N7(G)N7(G) adducts with DNA, causing a local distortion of a DNA strand. As a result, gel mobility of the DNA changes. Both AH3 and AH7 are found to be less active than cisplatin against the three cell lines with AH3 being the more active compound of the two. The higher activity of AH3 is in line with its lower molar conductivity value corresponding to a lower degree of dissociation.

Conclusion The differences in activity of AH3, AH7 and cisplatin against the cell lines illustrate structure-activity relationship.
Keyword Cis-planaramineplatinum
Cancer
Plasmid DNA
Ovarian cancer
Cisplatin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
 
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Created: Wed, 06 Jan 2010, 15:28:12 EST by Macushla Boyle on behalf of Faculty of Science