Immunocytochemical analysis of D-serine distribution in the mammalian brain reveals novel anatomical compartmentalizations in glia and neurons

Williams, Susan M., Diaz, Claudia M., Macnab, Lauren T., Sullivan, Robert K. P. and Pow, David V. (2006) Immunocytochemical analysis of D-serine distribution in the mammalian brain reveals novel anatomical compartmentalizations in glia and neurons. Glia, 53 4: 401-411. doi:10.1002/glia.20300


Author Williams, Susan M.
Diaz, Claudia M.
Macnab, Lauren T.
Sullivan, Robert K. P.
Pow, David V.
Title Immunocytochemical analysis of D-serine distribution in the mammalian brain reveals novel anatomical compartmentalizations in glia and neurons
Formatted title
Immunocytochemical analysis of D-serine distribution in the mammalian brain reveals novel anatomical compartmentalizations in glia and neurons
Journal name Glia   Check publisher's open access policy
ISSN 0894-1491
1098-1136
Publication date 2006-03
Sub-type Article (original research)
DOI 10.1002/glia.20300
Volume 53
Issue 4
Start page 401
End page 411
Total pages 11
Place of publication New York, U.S.A.
Publisher Wiley-Liss
Language eng
Subject 06 Biological Sciences
0601 Biochemistry and Cell Biology
1109 Neurosciences
Formatted abstract
D-Serine is a co-agonist at the NMDA receptor glycine-binding site. Early studies have emphasized a glial localization for D-serine. However the nature of the glial cells has not been fully resolved, because previous D-serine antibodies needed glutaraldehyde-fixation, precluding co-localization with fixation-sensitive antigens. We have raised a new D-serine antibody optimized for formaldehyde-fixation. Light and electron microscopic observations indicated that D-serine was concentrated into vesicle-like compartments in astrocytes and radial glial cells, rather than being distributed uniformly in the cytoplasm. In aged animals, patches of cortex and hippocampus were devoid of immunolabeling for D-serine, suggesting that impaired glial modulation of forebrain glutamatergic signaling might occur. Dual immunofluorescence labeling for glutamate and D-serine revealed D-serine in a subset of glutamatergic neurons, particularly in brainstem regions and in the olfactory bulbs. Microglia also contain D-serine. We suggest that some D-serine may be derived from the periphery. Collectively, our data suggest that the cellular compartmentation and distribution of D-serine may be more complex and extensive than previously thought and may have significant implications for our understanding of the role of D-serine in disease states including hypoxia and schizophrenia.
© 2005 Wiley-Liss, Inc.
Keyword Astrocyte
Glycine
Microglia
NMDA
Vesicle
Q-Index Code C1

 
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Created: Wed, 06 Jan 2010, 09:33:01 EST by Tara Johnson on behalf of Queensland Brain Institute