A novel family of carbohydrate-binding modules identified with Ruminococcus albus proteins

Xu, Qi, Morrison, Mark, Nelson, Karen E., Bayer, Edward, A., Atamna, Nof and Lamed, Raphael (2004) A novel family of carbohydrate-binding modules identified with Ruminococcus albus proteins. Febs Letters, 566 1-3: 11-16.


Author Xu, Qi
Morrison, Mark
Nelson, Karen E.
Bayer, Edward, A.
Atamna, Nof
Lamed, Raphael
Title A novel family of carbohydrate-binding modules identified with Ruminococcus albus proteins
Journal name Febs Letters   Check publisher's open access policy
ISSN 0014-5793
Publication date 2004-05
Sub-type Article (original research)
DOI 10.1016/j.febslet.2004.04.005
Volume 566
Issue 1-3
Start page 11
End page 16
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elseveir
Language eng
Subject 0601 Biochemistry and Cell Biology
0605 Microbiology
Abstract We recently showed that some of the enzymes underpinning cellulose solubilization by Ruminococcus albus 8 lack the conventional type of dockerin module characteristic of cellulosomal proteins and instead, bear an “X” domain of unknown function at their C-termini. We have now subcloned and expressed six X domains and showed that five of them bind to xylan, chitin, microcrystalline and phosphoric-acid swollen cellulose, as well as more heterogenous substrates such as alfalfa cell walls, banana stem and wheat straw. The X domain that did not bind to these substrates was derived from a family-5 glycoside hydrolase (Cel5G), which possesses two X domains in tandem. Whereas the internal X domain failed to bind to the substrates, the recombinant dyad exhibited markedly enhanced binding relative to that observed for the C-terminal X domain alone. The evidence supports a distinctive carbohydrate-binding role of broad specificity for this type of domain, and we propose a novel family (designated family 37) of carbohydrate-binding modules that appear to be peculiar to R. albus.
Keyword Ruminococcus albus
Carbohydrate binding molecules
Proteins
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 24 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 28 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Access Statistics: 56 Abstract Views  -  Detailed Statistics
Created: Mon, 04 Jan 2010, 15:05:28 EST by Elissa Saffery on behalf of Faculty of Science