Functional analysis of a cellular microRNA in insect host-ascovirus interaction

Hussain, Mazher and Asgari, Sassan (2010) Functional analysis of a cellular microRNA in insect host-ascovirus interaction. Journal of Virology, 84 1: 612-620. doi:10.1128/JVI.01794-09


Author Hussain, Mazher
Asgari, Sassan
Title Functional analysis of a cellular microRNA in insect host-ascovirus interaction
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1070-6321
1098-5514
Publication date 2010-01-01
Year available 2010
Sub-type Article (original research)
DOI 10.1128/JVI.01794-09
Open Access Status DOI
Volume 84
Issue 1
Start page 612
End page 620
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2011
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
060506 Virology
Formatted abstract
MicroRNAs (miRNAs) have emerged as key regulators in many biological processes, from development to defense, at almost all organismal levels. Recently, their role has been highlighted in pathogen-host interactions. Emerging evidence from a variety of virus-host systems indicates that cellular as well as virally encoded miRNAs influence viral replication. Here, we report changes in expression levels of host miRNAs upon ascovirus infection in an insect cell line and investigated the role of a host miRNA, Hz-miR24, in the host-virus system. We found that Hz-miR24 is differentially expressed following virus infection, with an increase in its expression levels late in infection. Experimental evidence demonstrated that Hz-miR24 downregulates ascoviral DNA-dependent RNA polymerase and its β subunit transcript levels late in infection. The specific miRNA-target interactions were investigated and confirmed using the ectopic expression of Hz-miR24 and a green fluorescent protein-based reporter system. Further, the expression of the target gene was substantially enhanced in cells transfected with a synthesized inhibitor of Hz-miR24. These findings suggest that ascoviruses manipulate host miRNAs that in turn regulate the expression of their genes at specific time points after infection. To our knowledge, this is the first cellular miRNA reported to interact with an insect virus.
Keyword Virus replication
Viral replication
Sequence
Expression
Targets
Ichnoviruses
Iridoviruses
Oorganisation
Recognition
Evolution
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2011 Collection
School of Biological Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 34 times in Scopus Article | Citations
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Created: Sun, 27 Dec 2009, 10:01:35 EST