Early molecular responses of coral larvae to hyperthermal stress

Rodriguez-Lanetty, M, Harii, S and Hoegh-Guldberg, O (2009) Early molecular responses of coral larvae to hyperthermal stress. Molecular Ecology, 18 24: 5101-5114. doi:10.1111/j.1365-294X.2009.04419.x

Author Rodriguez-Lanetty, M
Harii, S
Hoegh-Guldberg, O
Title Early molecular responses of coral larvae to hyperthermal stress
Journal name Molecular Ecology   Check publisher's open access policy
ISSN 0962-1083
Publication date 2009-12
Sub-type Article (original research)
DOI 10.1111/j.1365-294X.2009.04419.x
Open Access Status
Volume 18
Issue 24
Start page 5101
End page 5114
Total pages 14
Editor Loren Rieseberg
Place of publication Oxford, England, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2010
Language eng
Subject C1
9603 Climate and Climate Change
060205 Marine and Estuarine Ecology (incl. Marine Ichthyology)
Formatted abstract
Most of the work on the impact of elevated temperature and light on Symbiodiniuminvertebrate
symbioses have focused primarily on how the photosynthetic (algal) partner
is impacted. Understanding how the same stresses affect the invertebrate host, however,
is in its infancy. In this study, we re-examined the direct effect of elevated temperatures
on the invertebrate host exploring the early transcriptional response of aposymbiotic
(without algal symbionts) coral larvae. The temperatures tested in the experimental
design were 24 C (ambient seawater temperature), 28 C and 31 C; and the sampling
points were 3 and 10 h after temperature exposure. We explored relative changes in
transcription using a cDNA microarray constructed for the scleractinian coral, Acropora
, and containing 18 142 expressed sequence tag (EST) clones ⁄ 8386 unigenes.
Our study identified 29 genes that were significantly up- and down-regulated when
A. millepora coral larvae were exposed to elevated temperatures. Down-regulation of
several key components of DNA⁄RNA metabolism was detected implying inhibition of
general cellular processes. The down-regulation of protein synthesis, however, was not
simple and random, which suggested that the stress response was a more complicated
adjustment of cellular metabolism. We identified four significant outcomes during the
very early hours of the transcriptional response to hyperthermal stress in coral larvae.
First, the expression of heat-shock proteins increased rapidly (within 3 h) in response to
hyperthermal stress. Second, a fluorescent protein homologue, DsRed-type FP, decreased
its expression in response to elevated temperature reinforcing a potential role as a
molecular marker for monitoring hyperthermal stress in nature. Third, the downregulation
of a coral mannose-binding C-type lectin under elevated temperature suggests
that heat stress might compromise some components of the coral immune defence and
therefore might bring about susceptibility to pathogenic diseases. And last, genes
involved in protecting cells against oxidative stress showed little response at the early
hours to heat stress, supporting the proposal that up-regulation of cnidarian host oxidative
stress genes may require reactive oxygen species generated by stressed algal symbionts.
Keyword Climate change
Coral larvae
Coral stress response
Ecological genomics
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Centre for Marine Studies Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 80 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 83 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 27 Dec 2009, 00:01:10 EST