An extracellular matrix microarray for probing cellular differentiation

Flaim, Christopher J., Chien, Shu and Bhatia, Sangeeta N. (2005) An extracellular matrix microarray for probing cellular differentiation. Nature Methods, 2 2: 119-125. doi:10.1038/NMETH736

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Author Flaim, Christopher J.
Chien, Shu
Bhatia, Sangeeta N.
Title An extracellular matrix microarray for probing cellular differentiation
Journal name Nature Methods   Check publisher's open access policy
ISSN 1548-7091
Publication date 2005-02
Sub-type Article (original research)
DOI 10.1038/NMETH736
Volume 2
Issue 2
Start page 119
End page 125
Total pages 7
Place of publication New York, U.S.
Publisher Nature Publishing Group
Language eng
Subject 10 Technology
1007 Nanotechnology
Abstract We present an extracellular matrix (ECM) microarray platform for the culture of patterned cells atop combinatorial matrix mixtures. This platform enables the study of differentiation in response to a multitude of microenvironments in parallel. The fabrication process required only access to a standard robotic DNA spotter, off-the-shelf materials and 1,000 times less protein than conventional means of investigating cell-ECM interactions. To demonstrate its utility, we applied this platform to study the effects of 32 different combinations of five extracellular matrix molecules (collagen I, collagen III, collagen IV, laminin and fibronectin) on cellular differentiation in two contexts: maintenance of primary rat hepatocyte phenotype indicated by intracellular albumin staining and differentiation of mouse embryonic stem (ES) cells toward an early hepatic fate, indicated by expression of a beta-galactosidase reporter fused to the fetal liver-specific gene, Ankrd17 (also known as gtar). Using this technique, we identified combinations of ECM that synergistically impacted both hepatocyte function and ES cell differentiation. This versatile technique can be easily adapted to other applications, as it is amenable to studying almost any insoluble microenvironmental cue in a combinatorial fashion and is compatible with several cell types
Keyword Beta-galactosidase
Mouse Embryos
Embryonic stem cells
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Centre for Nanotechnology and Biomaterials Publications
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Citation counts: TR Web of Science Citation Count  Cited 495 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 526 times in Scopus Article | Citations
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Created: Thu, 17 Dec 2009, 14:34:04 EST by Ms May Balasaize on behalf of Faculty of Science