Adenosine stimulates connexin 43 expression and gap junctional communication in pituitary folliculostellate cells

Lewis, B. Mary, Pexa, Annette, Francis, Karen, Verma, Vandana, McNicol, Anne M., Scanlon, Maurice, Deussen, Andreas, Evans, W. Howard, Rees, D. Aled and Ham, Jack (2006) Adenosine stimulates connexin 43 expression and gap junctional communication in pituitary folliculostellate cells. The FASEB Journal, 20 14: 2585-2587.


Author Lewis, B. Mary
Pexa, Annette
Francis, Karen
Verma, Vandana
McNicol, Anne M.
Scanlon, Maurice
Deussen, Andreas
Evans, W. Howard
Rees, D. Aled
Ham, Jack
Title Adenosine stimulates connexin 43 expression and gap junctional communication in pituitary folliculostellate cells
Journal name The FASEB Journal   Check publisher's open access policy
ISSN 0892-6638
1530-6860
Publication date 2006-12
Sub-type Article (original research)
DOI 10.1096/fj.06-6121fje
Volume 20
Issue 14
Start page 2585
End page 2587
Total pages 3
Place of publication Bethesda, MD USA
Publisher Federation of American Societies for Experimental Biology
Language eng
Subject 11 Medical and Health Sciences
Abstract Adenosine is known to stimulate interleukin (IL)-6 and vascular endothelial growth factor (VEGF) secretion from pituitary TtT/GF folliculostellate (FS) cells indicating that it is an important paracrine regulator of anterior pituitary function. This study demonstrates that rodent anterior pituitary cell lines produce extracellular adenosine that is able to increase intercellular gap junction communication in FS cells. Ecto-5'-nucleotidase (CD73), the enzyme that generates adenosine from AMP, was demonstrated by immunocytochemistry in ~20% of anterior pituitary cells, and some of these cells colocalized with prolactin and growth hormone. CD73 mRNA and protein were detected in GH3 and MMQ (somatotroph-lactotroph lineages) and TtT/GF cells, and enzyme activity was demonstrated by the conversion of exogenously added fluorescent ethenoAMP to ethenoadenosine. Adenosine production, as measured by HPLC, was detected in GH3 (1 µM/h) and MMQ (3 µM/h) but not in TtT/GF cells. Adenosine (EC50: 0.5µM) and NECA (universal adenosine receptor agonist; EC50 0.1 µM) stimulated connexin 43 (Cx43) mRNA and protein expression within 1–2 h in TtT/GF cells. Adenosine and NECA also stimulated gap junctional intercellular communication (as assessed by transmission of Alexa Fluor 488) by 6- to 8-fold in comparison with untreated TtT/GF cells. In cocultures of MMQ and TtT/GF cells, Cx43 expression in TtT/GF cells increased in proportion to the number of MMQ cells plated out. These data suggest that adenosine, formed locally in the anterior pituitary gland can stimulate gap junction communication in FS cells.—Lewis, B. M., Pexa, A., Francis, K., Verma, V., McNicol, A. M., Scanlon, M., Deussen, A., Evans, W. H., Rees, D. A., Ham, J. Adenosine stimulates connexin 43 expression and gap junctional communication in pituitary FS cells.
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Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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