Protection from Pneumonic Infection with Burkholderia Species by Inhalational Immunotherapy

Goodyear, Andrew, Kellihan, Lisa, Bielefeldt-Ohmann, Helle, Troyer, Ryan, Propst, Katie and Dow, Steven (2009) Protection from Pneumonic Infection with Burkholderia Species by Inhalational Immunotherapy. Infection and Immunity, 77 4: 1579-1588. doi:10.1128/IAI.01384-08

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ189505_OA.pdf Full text (open access) application/pdf 1000.58KB 0

Author Goodyear, Andrew
Kellihan, Lisa
Bielefeldt-Ohmann, Helle
Troyer, Ryan
Propst, Katie
Dow, Steven
Title Protection from Pneumonic Infection with Burkholderia Species by Inhalational Immunotherapy
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 1098-5522
Publication date 2009-04
Year available 2009
Sub-type Article (original research)
DOI 10.1128/IAI.01384-08
Open Access Status File (Publisher version)
Volume 77
Issue 4
Start page 1579
End page 1588
Total pages 10
Editor Fang, Ferric C.
Place of publication Washington, DC, United States
Publisher American Society for Microbiology (ASM)
Collection year 2010
Language eng
Abstract Burkholderia mallei and B. pseudomallei are important human pathogens and cause the diseases glanders and melioidosis, respectively. Both organisms are highly infectious when inhaled and are inherently resistant to many antimicrobials, thus making it difficult to treat pneumonic Burkholderia infections. We investigated whether it was possible to achieve rapid protection against inhaled Burkholderia infection by using inhaled immunotherapy. For this purpose, cationic liposome DNA complexes (CLDC), which are potent activators of innate immunity, were used to elicit the activation of pulmonary innate immune responses. We found that mucosal CLDC administration before or shortly after bacterial challenge could generate complete or nearly complete protection from inhalational challenge with 100% lethal doses of B. mallei and B. pseudomallei. Protection was found to be dependent on the CLDC-mediated induction of gamma interferon responses in lung tissues and was partially dependent on the activation of NK cells. However, CLDC-mediated protection was not dependent on the induction of inducible nitric oxide synthase, as assessed by depletion studies. We concluded that the potent local activation of innate immune responses in the lung could be used to elicit rapid and nonspecific protection from aerosol exposure to both B. mallei and B. pseudomallei.
Keyword Burkholderia mallei
B. Pseudomallei
Antimicrobial Activities
Pneumonic Burkholderia Infections
Inhaled immunotheraphy
Cationic Liposomes
DNA Complexes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Veterinary Science Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 32 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 31 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 09 Dec 2009, 11:18:10 EST by Rosalind Blair on behalf of School of Veterinary Science